Constitutive expression of AtSINA2 from Arabidopsis improves grain yield, seed oil and drought tolerance in transgenic soybean
Constitutive expression of AtSINA2 from Arabidopsis improves grain yield, seed oil and drought tolerance in transgenic soybean
复制标题
拟南芥AtSINA2的组成型表达可提高转基因大豆的谷物产量、种子油和耐旱性
DOI：
10.1016/j.plaphy.2023.01.051
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发表时间：
2023-02-07
影响因子：
6.5
通讯作者：
Zhang，Hongxia
中科院分区：
文献类型：
--
作者：
Yang，Jin;Mao，Tingting;Zhang，Hongxia
7 IN absent （SINA）是环E3连接酶家族的一个典型成员，在植物生长发育和对非生物胁迫的响应中起着至关重要的作用。但其在油料作物中的生物学功能尚不清楚。此前，我们报道了atsina2在拟南芥中的过表达正调控转基因植物的抗旱性。本研究证明，atsina2基因在大豆中的异位表达提高了转基因植株的茎部生长、籽粒产量、耐旱性和种子含油量。与野生型相比，转基因大豆的茎部生物量和籽粒产量更高，籽粒耐油性和抗旱性也有所提高。生理分析表明，转基因植株抗旱性的提高伴随着叶绿素含量的增加，以及干旱胁迫下丙二醛积累和水分损失的减少。进一步的转录组学分析表明，在正常和干旱胁迫条件下，转基因大豆中与植物生长、开花和胁迫反应相关的基因表达均上调或下调。研究结果表明，atsina2可提高农业产量和抗旱性，可作为大豆高产抗旱新品种基因工程的候选基因。
The SEVEN IN Absentia (SINA), a typical member of the RING E3 ligase family, plays a crucial role in plant growth, development and response to abiotic stress. However, its biological functions in oil crops are still unknown. Previously, we reported that overexpression ofAtSINA2in Arabidopsis positively regulated the drought tolerance of transgenic plants. In this work, we demonstrate that ectopic expression ofAtSINA2in soybean improved the shoot growth, grain yield, drought tolerance and seed oil content in transgenic plants. Compared to wild type, transgenic soybean produced greater shoot biomass and grain yield, and showed improved seed oil and drought tolerance. Physiological analyses exhibited that the increased drought tolerance of transgenic plants was accompanied with a higher chlorophyll content, and a lower malondialdehyde accumulation and water loss during drought stress. Further transcriptomic analyses revealed that the expressions of genes related to plant growth, flowering and stress response were up- or down-regulated in transgenic soybean under both normal and drought stress conditions. Our findings imply thatAtSINA2improved both agricultural production and drought tolerance, and it can be used as a candidate gene for the genetic engineering of new soybean cultivars with improved grain yield and drought resistance.