En-masse production of elite clones of Dendrobium crepidatum: A threatened, medicinal orchid used in Traditional Chinese Medicine(TCM)
En-masse production of elite clones of Dendrobium crepidatum: A threatened, medicinal orchid used in Traditional Chinese Medicine(TCM)
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DOI：
10.1016/j.jarmap.2016.04.001
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发表时间：
2016-12-01
影响因子：
3.9
通讯作者：
Tandon, Pramod
中科院分区：
文献类型：
--
作者：
Bhattacharyya, Paromik;Kumaria, Suman;Tandon, Pramod
兰花是一种很有前途的药用植物，铁皮石斛是其中的重要药用植物。Lindl & Paxton因其多方向的药用属性而突出。本研究建立了一套以杜氏木茎节段为外植体的离体再生体系。crepidatum，以满足可持续的商业开发和保护需求。噻苯隆（TDZ）3 mg/l单独处理的反应频率为55%，与NAA 0.5mg/l和TDZ 2 mg/l组合处理的反应频率可提高到97%。多胺的使用进一步提高了不定芽的诱导率，在0.8mM腐胺沿着、TDZ 2 mg/l和NAA 0.5mg/l的培养基中，每外植体可获得11.8个不定芽。在IBA浓度为2 mg/l的培养基上生根率最高。利用起始密码子靶向（SCoT）多态性和简单重复序列间（ISSR）分析了驯化植株的遗传稳定性。SCoT揭示了10%的微繁殖植物内的总变异性，而累积的ISSR和SCoT数据显示6.25%的克隆变异性，表明高的遗传保真度之间的再生。利用DPPH和FRAP分析揭示了与母株相比，次生代谢产物沿着的全面更高的产量以及显著更高的抗氧化潜力。再生频率增加，次生代谢产物产量相对较高，遗传稳定性报告在目前的通信，确保独创性的克隆繁殖协议开发的D。其可进一步用于可持续商业利用和保护其他药用重要兰花物种。(C)2016年爱思唯尔有限公司。All rights reserved.
Orchids are one of the promising medicinal plant families of which Dendrobium crepidatum. Lindl & Paxton figures out prominently because of its multi directional medicinal attributes. In the present report, in vitro regeneration protocol has been developed from the nodal segments of D. crepidatum, to cater sustainable commercial exploitation and conservation needs. Thidiazuron (TDZ) at 3mg/l singly resulted in the response frequency of 55% which could be increased to 97% by incorporating NAA at 0.5mg/land TDZ at 2mg/l in combination in the medium. Shoot induction rate was further enhanced with the use of polyamines and at 0.8 mM putrescine along with 2mg/l TDZ and 0.5mg/l NAA in the medium11.8 shoots/ explant could be obtained. Highest rooting frequency of the shoots was achieved in medium containing 2mg/l IBA. Genetic stability of the acclimatized plants was assessed using Start Codon Targeted( SCoT) polymorphism and inters simple sequence repeats (ISSR). SCoT revealed a total variability of 10% within the micropropagated plants whereas the cumulative ISSR and SCoT data revealed 6.25% clonal variability indicating high genetic fidelity amongst the regenerates. A comprehensive higher yield of the secondary metabolites along with significant higher antioxidant potentials as compared to the mother plant was revealed using DPPH and FRAP assays. An increased regeneration frequency with a comparative higher yield of secondary metabolite and genetic stability reported in the present communication ensure the ingenuity of this clonal propagation protocol developed for D. crepidatum which can be further utilized in the sustainable commercial utilization and conservation of other medicinally important orchid species. (C) 2016 Elsevier GmbH. All rights reserved.