Reversible on-demand cell alignment using reconfigurable microtopography
Reversible on-demand cell alignment using reconfigurable microtopography
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DOI：
10.1016/j.biomaterials.2007.12.010
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发表时间：
2008-04-01
期刊：
影响因子：
14
通讯作者：
Takayama, Shuichi
中科院分区：
文献类型：
--
作者：
Lam, Mai T.;Clem, William C.;Takayama, Shuichi
传统的细胞培养基质由静态、平坦的表面组成，尽管在体内，细胞存在于各种动态地形上。我们报告了一个可重构的微地形系统的发展与细胞培养，是由可逆的波浪形的微特征聚（二甲基硅氧烷）兼容。通过首先等离子体氧化基底以在表面上产生薄的脆性膜，然后施加和释放压缩应变以分别引入和去除微特征，在细胞培养定制基底上诱导波状微图案的稳健可逆性。可逆的拓扑结构能够在24小时的间隔内在相同的基底上重复对齐、不对齐和重新对齐C2C12肌原性细胞系细胞，并且不抑制细胞分化。这里提出的材料和方法的灵活性和简单性提供了广泛适用的能力，通过该能力来研究和比较使用常规体外培养基质尚不容易研究的动态细胞过程。(c)2007爱思唯尔有限公司保留所有权利。
Traditional cell culture substrates consist of static, flat surfaces although in vivo, cells exist on various dynamic topographies. We report development of a reconfigurable microtopographical system compatible with cell culture that is comprised of reversible wavy microfeatures on poly(dimethylsiloxane). Robust reversibility of the wavy micropattern is induced on the cell culture customized substrate by first plasma oxidizing the substrate to create a thin, brittle film on the surface and then applying and releasing compressive strain, to introduce and remove the microfeatures, respectively. The reversible topography was able to align, unalign, and realign C2C12 myogenic cell line cells repeatedly on the same substrate within 24 h intervals, and did not inhibit cell differentiation. The flexibility and simplicity of the materials and methods presented here provide a broadly applicable capability by which to investigate and compare dynamic cellular processes not yet easily studied using conventional in vitro culture substrates. (c) 2007 Elsevier Ltd. All rights reserved.