Heterogeneity of Borrelia burgdorferi in the skin
Heterogeneity of Borrelia burgdorferi in the skin
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DOI：
10.1097/00000372-199612000-00004
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发表时间：
1996-12-01
影响因子：
1.1
通讯作者：
Jurecka, W
中科院分区：
文献类型：
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作者：
Aberer, E;Kersten, A;Jurecka, W
各种体外技术鉴定伯氏疏螺旋体感染的可靠性仍然不令人满意。使用高功率分辨率的视频显微镜和染色与疏螺旋体属特异性单克隆鞭毛抗体H9724，我们确定了疏螺旋体结构的皮肤活检慢性游走性红斑（疏螺旋体后来培养），慢性萎缩性肢端皮炎，和硬斑病。除了典型的疏螺旋体，我们注意到不同形状的染色结构与“疏螺旋体注射皮肤”模型中发现的疏螺旋体相同;与琼脂包埋的疏螺旋体相同;与暴露于超免疫血清和/或抗生素后培养的疏螺旋体相同。我们的结论是，H9724反应性结构代表了各种形式的B。burgdorferi而不是染色文物。这些“非典型”形式的B。burgdorferi可能代表该细菌的体内形态学变体。
The reliability of various in vitro techniques to identify Borrelia burgdorferi infection is still unsatisfactory. Using a high-power resolution videomicroscope and staining with the borrelia genus-specific monoclonal flagellar antibody H9724, we identified borrelial structures in skin biopsies of erythema chronicum migrans (from which borrelia later was cultured), of acrodermatitis chronica atrophicans, and of morphea. In addition to typical borreliae, we noted stained structures of varying shapes identical to borreliae found in a ''borrelia-injected skin'' model; identical to agar-embedded borreliae; and identical to cultured borreliae following exposure to hyperimmune sera and/or antibiotics. We conclude that the H9724-reactive structures represent various forms of B. burgdorferi rather than staining artifacts. These ''atypical'' forms of B. burgdorferi may represent in vivo morphologic variants of this bacterium.