Lymphatic mapping of mice with systemic lymphoproliferative disorder: Usefulness as an inter-lymph node metastasis model of cancer
Lymphatic mapping of mice with systemic lymphoproliferative disorder: Usefulness as an inter-lymph node metastasis model of cancer
复制标题
DOI：
10.1016/j.jim.2013.01.004
复制
发表时间：
2013-03-01
影响因子：
2.2
通讯作者：
Kodama, Tetsuya
中科院分区：
文献类型：
--
作者：
Shao, Lenan;Mori, Shiro;Kodama, Tetsuya
淋巴结转移的临床前模型是研究和设计诊断和治疗淋巴结转移的新技术的基础。然而，由于正常小鼠的LN直径为1-2 mm，因此使用传统成像方式识别小鼠的LN和淋巴管（LV）具有挑战性。在这里，我们描述了MXH 10/ Mo-lpr/lpr（MXH 10/Mo/lpr）近交系小鼠，它们的LN出现全身肿胀，直径高达10 mm，从而可以研究LN和LV的地形。使用大体解剖解剖方法，我们确定了22个不同的淋巴结位于头颈部，四肢，胸部和腹部区域。此外，还发现了四条外周LN间血管：从髂下LN（SiLN）到腋窝固有LN（PALN）;从腮腺LN到尾侧颈深LN;以及从腘LN到坐骨LN和SiLN。通过LVs向PALN的转移是通过将PMFM 3A/Luc小鼠乳腺癌细胞诱导到SiLN中。我们的研究结果表明，MXH 10/Mo/lpr小鼠品系是一个很好的模型，其中研究淋巴引流和LN间转移的癌症。本文揭示了小鼠淋巴管的解剖结构，为研究癌症的LN间转移，特别是癌细胞通过LN间血管运输的机制提供了新的见解。这些结果提供的数据可能在寻求开发更好的基于淋巴引流的药物递送系统方面非常有用。(C)2013爱思唯尔有限公司版权所有。
Preclinical models of lymph node (LN) metastasis are fundamental to the study and design of new techniques for the diagnosis and treatment of LN metastasis. However, the identification of LNs and lymphatic vessels (LVs) in mice is challenging with conventional imaging modalities, since the LN diameter in normal mice is 1-2 mm. Here, we describe MXH10/ Mo-lpr/lpr (MXH10/Mo/lpr) inbred mice, which develop systemic swelling of LNs up to 10 mm in diameter, allowing investigation of the topography of LNs and LVs. Using a gross anatomy dissection approach, we identified 22 different LNs situated in the head and neck, limbs, thoracic and abdominal regions. Furthermore, four peripheral inter-LN vessels were found: from the subiliac LN (SiLN) to the proper axillary LN (PALN); from the parotid LN to the caudal deep cervical LN; and from the popliteal LN to both the sciatic LN and the SiLN. Metastasis to the PALN via LVs was induced by inoculating FM3A/Luc mouse mammary carcinoma cells into the SiLN. Our results demonstrate that the MXH10/Mo/lpr mouse strain is an excellent model in which to investigate lymphatic drainage and inter-LN metastasis of cancer. This paper unveils the anatomy of murine lymphatics to give new insights into the investigation of inter-LN metastasis of cancer, especially the mechanisms involved in the trafficking of cancer cells through inter-LN vessels. The results provide data that may prove very useful in the quest to develop better lymph drainage-based drug delivery systems. (C) 2013 Elsevier B.V. All rights reserved.