Transcriptome analysis of the growth performance of hybrid mandarin fish after food conversion.
Transcriptome analysis of the growth performance of hybrid mandarin fish after food conversion.
复制标题
DOI：
10.1371/journal.pone.0240308
复制
发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Feng-Liu
中科院分区：
文献类型：
--
作者：
Guan WZ;Qiu GF;Feng-Liu
近年来，中国已成为鳜鱼驯化的热点，具有巨大的商业价值。尽管人们已经研究了驯养鳜鱼的食物偏好，但对于调节其生长的基因却知之甚少。我们用人工饲料饲养杂交鳜鱼3个月，结果表明，杂交鳜鱼的成活率为60.00%。它们的总长度和体重分别为18.34±0.43厘米和100.44±4.87克。绝对身长和体重增加率分别为 0.14 厘米/天和 1.08 克/天。最后，进行 RNA 测序 (RNA-Seq) 来识别响应生长性能而激活的潜在基因和途径。转录组分析生成了 68 个、197 个转录本和 45,871 个单基因。其中，快生长鱼和慢生长鱼之间有1025个基因上调，593个基因下调。最终获得32个差异表达基因，主要与脂肪酸生物合成（如FASN和ACACB）、集合管酸分泌（如ATP6E和KCC4）、细胞周期（如CDC20和CCNB）以及胰岛素样生长因子（IGF）系统（IGFBP1）相关。这些途径可能与杂交鳜鱼的生长有关。此外，在快速生长的鱼类中比在缓慢生长的鱼类中检测到更多潜在的单核苷酸多态性（SNP）。结果表明，新陈代谢和丰富等位基因的相互作用可能决定食物转换后杂交鳜鱼的生长。
During recent years, China has become a hotspot for the domestication of mandarin fish, and this is of great commercial value. Although the food preference of domesticated mandarin fish has been studied, little is known about genes regulating their growth. We raised hybrid mandarin fish on artificial feed for 3 months, the results showed that the survival rate of hybrid mandarin fish was 60.00%. Their total length and body weight were 18.34 ±0.43 cm and 100.44 ±4.87 g. The absolute length and weight gain rates were 0.14 cm/d and 1.08 g/d, respectively. Finally, RNA sequencing (RNA-Seq) was performed to identify potential genes and pathways activated in response to growth performance. The transcriptome analysis generated 68, 197 transcripts and 45,871 unigenes. Among them, 1025 genes were up-regulated and 593 genes were down-regulated between the fast- and slow-growth fish. Finally, we obtained 32 differentially expressed genes, which were mainly related to fatty acid biosynthesis (e.g. FASN and ACACB), collecting duct acid secretion (e.g. ATP6E and KCC4), cell cycle (e.g. CDC20 and CCNB), and the insulin-like growth factor (IGF) system (IGFBP1). These pathways might be related to the growth of hybrid mandarin fish. In addition, more potential single nucleotide polymorphisms (SNPs) were detected in the fast-growth fish than in the slow-growth fish. The results suggest that the interaction of metabolism and abundant alleles might determine the growth of hybrid mandarin fish after food conversion.