RECOGNITION BY ELAM-1 OF THE SIALYL-LEX DETERMINANT ON MYELOID AND TUMOR-CELLS
RECOGNITION BY ELAM-1 OF THE SIALYL-LEX DETERMINANT ON MYELOID AND TUMOR-CELLS
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DOI：
10.1126/science.1701275
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发表时间：
1990-11-23
期刊：
影响因子：
56.9
通讯作者：
SEED, B
中科院分区：
文献类型：
--
作者：
WALZ, G;ARUFFO, A;SEED, B
内皮细胞白细胞粘附分子-1（ELAM-1）是一种内皮细胞粘附分子，可使骨髓细胞粘附于炎症部位附近的血管壁。ELAM-1识别唾液酸-路易斯X（sialyl-LeX）决定簇NeuAc α 2 - 3Gal β 1 -4（Fuc.alpha.1-3）GlcNAc-，一种也在某些肿瘤细胞系表面上发现的粒细胞碳水化合物。髓样细胞与可溶性ELAM-1的结合被识别唾液酸LeX的单克隆抗体或携带唾液酸LeX的蛋白质抑制，其中一些可能参与髓样细胞粘附的体液调节。受刺激的粒细胞也释放ELAM-1结合的抑制剂，其可以被唾液酸-LeX的单克隆抗体选择性吸附。
Endothelial leukocyte adhesion molecule-1 (ELAM-1) is an endothelial cell adhesion molecule that allows myeloid cells to attach to the walls of blood vessels adjacent to sites of inflammation. ELAM-1 recognizes the sialyl-Lewis X (sialyl-LeX) determinant, NeuAc.alpha.2-3Gal.beta.1-4(Fuc.alpha.1-3)GlcNAc-, a granulocyte carbohydrate also found on the surface of some tumor cell lines. Binding of myeloid cells to soluble ELAM-1 is inhibited by a monoclonal antibody recognizing sialyl-LeX or by proteins bearing sialyl-LeX, some of which may participate in humoral regulation of myeloid cell adhesion. Stimulated granulocytes also release an inhibitor of ELAM-1 binding that can be selectively adsorbed by monoclonal antibody to sialyl-LeX.