A G-PROTEIN LINKED RECEPTOR FOR PARATHYROID-HORMONE AND PARATHYROID-HORMONE RELATED PEPTIDE
A G-PROTEIN LINKED RECEPTOR FOR PARATHYROID-HORMONE AND PARATHYROID-HORMONE RELATED PEPTIDE
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DOI：
10.1126/science.1658941
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发表时间：
1991-11-15
期刊：
影响因子：
56.9
通讯作者：
SEGRE, GV
中科院分区：
文献类型：
--
作者：
JUPPNER, H;ABOUSAMRA, AB;SEGRE, GV
使用负鼠肾细胞互补DNA（cDNA）库，通过COS-7表达克隆了编码585个氨基酸的甲状旁腺激素-甲状旁腺激素相关肽（PTH-PTHrP）受体的互补DNA，该受体具有7个潜在的跨膜结构域。表达的受体以相等的亲和力结合PTH和PTHrP，并且两种配体等效地刺激腺苷酸环化酶。惊人的同源性与降钙素受体和缺乏同源性与其他G蛋白连接的受体表明，这些钙调节激素的受体是相关的，并代表一个新的家庭。
The complementary DNA encoding a 585-amino acid parathyroid hormone-parathyroid hormone-related peptide (PTH-PTHrP) receptor with seven potential membrane-spanning domains was cloned by COS-7 expression using an opossum kidney cell complementary DNA (cDNA) library. The expressed receptor binds PTH and PTHrP with equal affinity, and both ligands equivalently stimulate adenylate cyclase. Striking homology with the calcitonin receptor and lack of homology with other G protein-linked receptors indicate that receptors for these calcium-regulating hormones are related and represent a new family.