Cross-linked chitosan polymers as generic adsorbents for simultaneous adsorption of multiple mycotoxins
Cross-linked chitosan polymers as generic adsorbents for simultaneous adsorption of multiple mycotoxins
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DOI：
10.1016/j.foodcont.2015.05.014
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发表时间：
2015-11
期刊：
影响因子：
6
通讯作者：
Zhiyong Zhao;Na Liu;Lingchen Yang;Jianhua Wang;Suquan Song;Dongxia Nie;Xianli Yang;J. Hou;Aibo Wu
中科院分区：
文献类型：
--
作者：
Zhiyong Zhao;Na Liu;Lingchen Yang;Jianhua Wang;Suquan Song;Dongxia Nie;Xianli Yang;J. Hou;Aibo Wu
本研究的主要目的是合成三种类型的交联壳聚糖聚合物，并进一步研究其对多种真菌毒素的吸附能力，包括黄曲霉毒素B1（AFB 1），赭曲霉毒素A（OTA），玉米赤霉烯酮（ZEN），伏马菌素B1（FB 1），脱氧雪腐镰刀菌烯醇（DON）和T-2毒素（T2）。其中，交联壳聚糖-戊二醛复合物对AFB 1、OTA、ZEN和FB 1的吸附率最高，分别为73%、97%、94%和99%，而对DON和T2的吸附率不明显（<30%）。还研究了各种孵育条件（接触时间、剂量和pH）的影响。随后，将实验数据拟合为Langmuir、Freundlich和Hill模型。描述AFB 1和FB 1吸附的最佳拟合模型为朗缪尔模型（R2≥ 0.99），AFB 1的理论最大吸附量为5.67 mg/g，FB 1的理论最大吸附量为15.7 mg/g。Hill模型对OTA和ZEN吸附的拟合效果最好（R2> 0.98），预测最大吸附量分别为24.8mg/g和9.18mg/g。此外，还在缓冲体系和模拟胃肠道条件下评价了吸附剂对多种真菌毒素同时存在的吸附能力。结果表明，多种真菌毒素共存不影响吸附剂的吸附能力，而某些胃肠成分则会降低毒素的吸附量。本研究的结果表明，壳聚糖-戊二醛复合物有可能被应用为多毒素吸附材料，以减少多种霉菌毒素对人类和动物的联合不良反应。
The primary objective of this study was to synthesize three types of cross-linked chitosan polymers and further investigate their adsorption capability for multiple mycotoxins, including aflatoxin B1(AFB1), ochratoxin A (OTA), zearalenone (ZEN), fumonisin B1(FB1), deoxynivalenol (DON) and T-2 toxin (T2). Among these synthetic adsorbents, cross-linked chitosan-glutaraldehyde complex presented the highest adsorption capability for AFB1(73%), OTA (97%), ZEN (94%) and FB1(99%), but no obvious adsorption for DON and T2 (<30%). The effect of various incubation conditions (contact time, dosage and pH) was also studied. Subsequently, the experimental data were fitted to Langmuir, Freundlich and Hill models. The best fitting model to describe AFB1and FB1adsorption was Langmuir model (R2≥ 0.99), with the theoretical maximum adsorption amounts of 5.67 mg/g for AFB1and 15.7 mg/g for FB1. The Hill model was the best model for OTA and ZEN adsorption (R2> 0.98), with the predicted maximum adsorption amounts were 24.8 mg/g for OTA and 9.18 mg/g for ZEN. In addition, the adsorption capability of adsorbent for the simultaneous presence of multiple mycotoxins was also evaluated in buffer system and simulated gastrointestinal condition. The results indicated that the coexisted multiple mycotoxins didn't affected the adsorption capability of adsorbent, whereas the adsorption amounts of toxins were decreased by some gastrointestinal components. The findings of this research suggest that chitosan–glutaraldehyde complex has the potential to be applied as multitoxin adsorbent material for reducing the combined adverse effect of multiple mycotoxins on humans and animals.