Treadmill running prevents atrophy differently in fast- versus slow-twitch muscles in a rat model of rheumatoid arthritis
Treadmill running prevents atrophy differently in fast- versus slow-twitch muscles in a rat model of rheumatoid arthritis
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DOI：
10.1007/s10974-021-09610-0
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发表时间：
2021-10-23
影响因子：
2.7
通讯作者：
Takahashi, Kenji
中科院分区：
文献类型：
--
作者：
Kamada, Yoichiro;Toyama, Shogo;Takahashi, Kenji
为探讨跑台运动对两种不同类型骨骼肌的影响，我们建立了胶原诱导性关节炎（CIA）大鼠模型。研究的骨骼肌是指长伸肌（EDL），它富含快肌纤维，比目鱼肌，它富含慢肌纤维。在免疫致敏后14和44天，这些肌肉的组织学和转录变化在被迫运动的大鼠（CIA前组）和自由饲养的CIA大鼠（CIA无组）之间进行了比较。在单次跑台跑步后第14天检查蛋白质表达的变化。跑台运动对每种肌肉类型的相对肌重、总截面积和纤维截面积有不同的影响。在比目鱼肌中，它防止了肌肉萎缩。转录分析显示，CIA前组比目鱼肌中，第14天真核翻译起始因子4 E（Eif 4 e）表达增加，第44天Atrogin-1和过氧化物酶体增殖物激活受体γ共激活因子1-α（PGC-1 α）表达增加，表明肌肉类型和运动之间存在相互作用。在第14天，单次跑步机跑步增加了比目鱼肌中Eif 4 e和p70 S6 K的水平，并降低了Atrogin-1的水平。在类风湿性关节炎的大鼠模型中，跑步机运动通过激活线粒体功能防止比目鱼肌的肌肉萎缩，如PGC-1 α表达增加所证明的。
To investigate the effects of treadmill running on two different types of skeletal muscle, we established a rat model of collagen-induced arthritis (CIA). The skeletal muscles studied were the extensor digitorum longus (EDL), which is rich in fast-twitch muscle fibers, and the soleus, which is rich in slow-twitch muscle fibers. The histological and transcriptional changes in these muscles at 14 and 44 days after immunosensitization were compared between rats that were forced to exercise (CIA ex group) and free-reared CIA rats (CIA no group). Change in protein expression was examined on day 14 after a single bout of treadmill running. Treadmill running had different effects on the relative muscle weight and total and fiber cross-sectional areas in each muscle type. In the soleus, it prevented muscle atrophy. Transcriptional analysis revealed increased eukaryotic translation initiation factor 4E (Eif4e) expression on day 14 and increased Atrogin-1 and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1 alpha) expression on day 44 in the soleus in the CIA ex group, suggesting an interaction between muscle type and exercise. A single bout of treadmill running increased the level of Eif4e and p70S6K and decreased that of Atrogin-1 in the soleus on day 14. Treadmill running prevented muscle atrophy in the soleus in a rat model of rheumatoid arthritis via activation of mitochondrial function, as evidenced by increased PGC-1 alpha expression.