Changes of Articular Cartilage after Immobilization in a Rat Knee Contracture Model
Changes of Articular Cartilage after Immobilization in a Rat Knee Contracture Model
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DOI：
10.1002/jor.20724
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发表时间：
2009-02-01
影响因子：
2.8
通讯作者：
Itoi, Eiji
中科院分区：
文献类型：
--
作者：
Hagiwara, Yoshihiro;Ando, Akira;Itoi, Eiji
目的：研究制动过程中大鼠膝关节软骨的变化。成年雄性大鼠膝关节在屈曲150度时用内固定器固定3d，1、2、4、8、16周。取膝关节中髁内侧区关节软骨，分为3个区(非接触区、过渡区、接触区)，通过大体观察、组织形态计量学分级及软骨细胞厚度和数量的测量，评价关节软骨退变程度。用扫描声学显微镜测量声速，评价关节软骨的弹性。关节软骨退变主要见于接触区和移行区。这两个区域的基质藏红素-O染色强度和软骨细胞数量均减少。非接触区和接触区关节软骨厚度无明显变化，而过渡区关节软骨厚度增加。在股骨和胫骨软骨的过渡区都观察到声速的降低，这表明关节软骨软化。关节软骨的改变早在制动后1周就开始明显。这些改变可能是由于制动过程中缺乏机械应力或关节液体循环不足所致。虽然我们不知道关节软骨这些变化的可逆性，但早期松动是避免这些软骨变化的较好方法。(C)2008年骨科研究会。由Wiley期刊公司出版，J Orthop Res 27：236-242,2009
The objective was to determine the changes of articular cartilage of the knee joint during immobilization in a rat model. The knee joints of adult male rats were immobilized at 150 degrees of flexion using an internal fixator for 3 days, and 1, 2, 4, 8, and 16 weeks. The articular cartilage from the medial midcondylar region of the knee was obtained, divided into three areas (non-contact area, transitional area, contact area), and in each area, a degree of degeneration was evaluated by gross observation, histomorphometric grading, and measurements of thickness and number of chondrocytes. Elasticity of the articular cartilage was estimated by measuring the sound speed with use of scanning acoustic microscopy. Degeneration of the articular cartilage was mainly observed in the contact and transitional areas. Matrix staining intensity by safranin-O and number of chondrocytes were decreased in these two areas. The thickness of the articular cartilage in the non-contact and contact areas was unchanged, but it was increased in the transitional area. Decrease in sound speed was observed in the transitional area of both the femoral and tibial cartilage, indicating the softening of the articular cartilage. The changes of articular cartilage became obvious as early as 1 week after immobilization. These changes may be due to a lack of mechanical stress or a lack of joint fluid circulation during immobilization. Although we do not know the reversibility of these changes of articular cartilage, early mobilization is preferable to avoid these cartilage changes. (C) 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc, J Orthop Res 27:236-242, 2009