Titanium dioxide nanoparticles enhanced thyroid endocrine disruption of pentachlorophenol rather than neurobehavioral defects in zebrafish larvae
Titanium dioxide nanoparticles enhanced thyroid endocrine disruption of pentachlorophenol rather than neurobehavioral defects in zebrafish larvae
复制标题
二氧化钛纳米粒子增强了五氯苯酚的甲状腺内分泌干扰，而不是斑马鱼幼虫的神经行为缺陷
DOI：
10.1016/j.chemosphere.2020.126536
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发表时间：
2020-06-01
期刊：
影响因子：
8.8
通讯作者：
Zhou, Bingsheng
中科院分区：
文献类型：
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作者：
Lei, Lei;Qiao, Kun;Zhou, Bingsheng
研究了纳米二氧化钛（n-TiO 2）对五氯酚（PCP）诱导的斑马鱼（Danio rerio）甲状腺内分泌紊乱和神经行为缺陷的影响。胚胎（受精后2小时）暴露于五氯苯酚（0、3、10和30微克/升）或与正二氧化钛（0.1毫克/升）的混合物中，直至受精后6天。结果表明，n-TiO 2单独使用不影响甲状腺激素水平或相关基因的转录。五氯苯酚暴露显著降低甲状腺激素甲状腺素（T4）含量、促甲状腺激素（TSH）水平和甲状腺球蛋白（tg）转录，但显著升高3，5，3 '-三碘甲状腺原氨酸（T3）水平和脱碘酶2（dio 2）上调。相比之下，与n-TiO 2的共暴露通过抑制潜在目标tg和d10 2而显著降低了T3的含量。对于神经毒性，单次和共同暴露导致神经发育相关基因（ELAV样RNA结合蛋白3，elavl 3;生长相关蛋白-43，gap 43; α-微管蛋白）显著下调并抑制运动活性的类似效应。结果表明，n-TiO 2的存在显著增强了PCP诱导的斑马鱼幼鱼甲状腺内分泌紊乱，但对神经行为缺陷无明显影响。(C)2020爱思唯尔有限公司保留所有权利。
This study investigated the influences of titanium dioxide nanoparticles (n-TiO2) on the thyroid endocrine disruption and neurobehavioral defects induced by pentachlorophenol (PCP) in zebrafish (Danio rerio). Embryos (2 h post-fertilization) were exposed to PCP (0, 3, 10, and 30 mu g/L) or in combination with n-TiO2 (0.1 mg/L) until 6 days post-fertilization. The results showed that n-TiO2 alone did not affect thyroid hormones levels or transcriptions of related genes. Exposure to PCP significantly decreased thyroid hormone thyroxine (T4) content, thyroid stimulating hormone (TSH) level and transcription of thyroglobulin (tg), but significantly increased 3,5,3'-triiodothyronine (T3) level and upregulation of deiodinase 2 (dio2). In comparison, the co-exposure with n-TiO2 significantly reduced the content of T3 by depressing the potential targets, tg and dio2. For neurotoxicity, the single and co-exposure resulted in similar effects with significant downregulation of neurodevelopment-related genes (ELAV like RNA Binding Protein 3, elavl3; Growth associated protein-43, gap43; alpha-tubulin) and inhibited locomotor activity. The results indicated that the presence of n-TiO2 significantly enhanced the PCP-induced thyroid endocrine disruption but not the neurobehavioral defects in zebrafish larvae. (C) 2020 Elsevier Ltd. All rights reserved.