Noncontact microsurgery and micromanipulation of living cells with combined system femtosecond laser scalpel-optical tweezers
Noncontact microsurgery and micromanipulation of living cells with combined system femtosecond laser scalpel-optical tweezers
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飞秒激光手术刀-光镊组合系统对活细胞进行非接触式显微手术和显微操作
DOI：
10.1117/12.922333
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发表时间：
2012
影响因子：
3.8
通讯作者：
M. Semenova
中科院分区：
文献类型：
--
作者：
I. V. Il’ina;D. Sitnikov;A. Ovchinnikov;M. Agranat;Y. Khramova;M. Semenova
我们报告了使用自行开发的飞秒激光手术刀（Cr：镁橄榄石种子振荡器和再生放大器，620 nm，100 fs，10 Hz）和光镊（连续激光，1064 nm）组成的组合激光系统进行非接触式激光介导的极体（PB）和滋养外胚层（TE）活检的早期哺乳动物胚胎的结果。为了进行PB活检，首先使用飞秒激光手术刀在透明质膜上钻孔，然后用光学镊子将PB从受精卵中取出。与PB活检不同，TE活检可以诊断母源性和父源性缺陷。此外，由于可以从胚胎中提取多个TE细胞，因此可以进行更可靠的诊断。通过施加激光脉冲以解剖在孵化期间刚刚离开透明膜的所需量的TE细胞来进行TE活检。然后使用光镊以规定的方式捕获和移动解剖的TE细胞。光镊中的激光功率和飞秒激光脉冲的能量被彻底优化，以防止细胞损伤并获得高的存活率。总之，所提出的基于激光的胚胎活检技术能够对活细胞进行准确、无污染、简单和快速的显微处理。
We report on the results of using self-developed combined laser system consisting of a femtosecond laser scalpel (Cr:Forsterite seed oscillator and a regenerative amplifier, 620 nm, 100 fs, 10 Hz) and optical tweezers (cw laser, 1064 nm) for performing noncontact laser-mediated polar body (PB) and trophectoderm (TE) biopsy of early mammalian embryos. To perform PB biopsy the femtosecond laser scalpel was initially used to drill an opening in the zona pellucida, and then the PB was extracted out of the zygote with the optical tweezers. Unlike PB biopsy, TE biopsy allows diagnosing maternally-derived as well as paternally-derived defects. Moreover, as multiple TE cells can be taken from the embryo, more reliable diagnosis can be done. TE biopsy was performed by applying laser pulses to dissect the desired amount of TE cells that had just left the zona pellucida during the hatching. Optical tweezers were then used to trap and move the dissected TE cells in a prescribed way. Laser power in optical tweezers and energy of femtosecond laser pulses were thoroughly optimized to prevent cell damage and obtain high viability rates. In conclusion, the proposed techniques of laser-based embryo biopsy enable accurate, contamination-free, simple and quick microprocessing of living cells.
影响因子：
3.7
作者：
LIANG, H;WRIGHT, WH;BERNS, MW
通讯作者：
BERNS, MW