Plasma Lactate Levels Increase during Hyperinsulinemic Euglycemic Clamp and Oral Glucose Tolerance Test
Plasma Lactate Levels Increase during Hyperinsulinemic Euglycemic Clamp and Oral Glucose Tolerance Test
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DOI：
10.1155/2015/102054
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发表时间：
2015-01-01
影响因子：
4.3
通讯作者：
Seyoum, Berhane
中科院分区：
文献类型：
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作者：
Berhane, Feven;Fite, Alemu;Seyoum, Berhane
胰岛素抵抗在2型糖尿病（T2D）的发病机制中起着核心作用，是预示T2D发生的早期指标。了解胰岛素抵抗早期细胞水平的代谢变化是非常必要的。本研究的目的是确定正常非糖尿病受试者在口服葡萄糖耐量试验（OGTT）和高胰岛素正葡萄糖钳夹（HIEC）研究期间循环乳酸水平的模式。22名受试者在OGTT和HIEC期间每30分钟测定一次乳酸和甘油。在整个HIEC研究期间，乳酸逐渐增加（P < 0.001）。基线时BMI 30的参与者< 30 had significantly higher mean M-values compared to those with BMI >（P < 0.05）。这一趋势在OGTT期间也一直持续。糖耐量异常组的平均乳酸水平显著高于糖耐量正常组（P < 0.001）。总之，我们发现在HIEC研究期间乳酸增加，这是一种高胰岛素血症状态，类似于T2D发展早期阶段观察到的代谢环境。
Insulin resistance, which plays a central role in the pathogenesis of type 2 diabetes (T2D), is an early indicator that heralds the occurrence of T2D. It is imperative to understand the metabolic changes that occur at the cellular level in the early stages of insulin resistance. The objective of this study was to determine the pattern of circulating lactate levels during oral glucose tolerance test (OGTT) and hyperinsulinemic euglycemic clamp (HIEC) study in normal nondiabetic subjects. Lactate and glycerol were determined every 30 minutes during OGTT and HIEC on 22 participants. Lactate progressively increased throughout the HIEC study period (P < 0.001). Participants with BMI < 30 had significantly higher mean M-values compared to those with BMI > 30 at baseline (P < 0.05). This trend also continued throughout the OGTT. In addition, those with impaired glucose tolerance test (IGT) had significantly higher mean lactate levels compared to those with normal glucose tolerance (P < 0.001). In conclusion, we found that lactate increased during HIEC study, which is a state of hyperinsulinemia similar to the metabolic milieu seen during the early stages in the development of T2D.