Association of Tissue mRNA and Serum Antigen Levels of Members of the Urokinase-Type Plasminogen Activator System with Clinical and Prognostic Parameters in Prostate Cancer
Association of Tissue mRNA and Serum Antigen Levels of Members of the Urokinase-Type Plasminogen Activator System with Clinical and Prognostic Parameters in Prostate Cancer
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DOI：
10.1155/2014/972587
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发表时间：
2014-01-01
影响因子：
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通讯作者：
Fuessel, Susanne
中科院分区：
文献类型：
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作者：
Al-Janabi, Omar;Taubert, Helge;Fuessel, Susanne
目的是分别测定前列腺癌 (PCa) 患者组织标本和血清样本中 uPA 系统成分的 mRNA 表达和蛋白水平，并评估其与临床病理学参数和总生存 (OS) 的关系。通过定量 PCR 分析 132 名 PCa 患者相应恶性和癌旁非恶性组织标本中 uPA、其受体 (uPAR) 及其抑制剂 1 型 (PAI-1) 的 mRNA 表达水平。通过 ELISA 分析 81 名 PCa 患者的术前血清样本中 uPA 系统成员的抗原水平。肿瘤组织中 uPA 系统成分的 RNA 水平显示出彼此之间的显着相关性。与相应的非恶性组织相比，PCa 中的 uPAmRNA 表达显着降低。高 uPA mRNA 水平与高格里森评分显着相关。血清中可溶性 uPAR (suPAR) 浓度升高与 PCa 患者的 OS 较差显着相关 (P = 0.022)。 suPAR 水平高的 PCa 患者死亡风险显着较高（多变量 Cox 回归分析；IIR - 7.12，P - 0.027）。高 suPAR 水平与 PCa 患者生存率低之间的关联提示癌症患者血清中 suPAR 水平对预后的影响。
The objective was to determine the mRNA expression and protein levels of uPA system components in tissue specimens and serum samples, respectively, from prostate cancer (PCa) patients and to assess their association with clinicopathological parameters and overall survival (OS). The mRNA expression levels of uPA, its receptor (uPAR), and its inhibitor type 1 (PAI-1) were analyzed in corresponding malignant and adjacent nonmalignant tissue specimens from 132 PCa patients by quantitative PCR. Preoperative serum samples from 81 PCa patients were analyzed for antigen levels of uPA system members by ELISA. RNA levels of uPA system components displayed significant correlations with each other in the tumor tissues. A significantly decreased uP AmRNA expression in PCa compared to the corresponding nonmalignant tissue was detected. High uPA mRNA level was significantly associated with a high Gleason score. Elevated concentration of soluble uPAR (suPAR) in serum was significantly associated with a poor OS of PCa patients (P = 0.022). PCa patients with high suPAR levels have a significantly higher risk of death (multivariate Cox's regression analysis; IIR - 7.12, P - 0.027). The association of high suPAR levels with poor survival of PCa patients suggests a prognostic impact of suPAR levels in serum of cancer patients.