LysR-type Transcriptional Regulator ChiR Is Essential for Production of All Chitinases and a Chitin-Binding Protein, CBP21, in Serratia marcescens 2170
LysR-type Transcriptional Regulator ChiR Is Essential for Production of All Chitinases and a Chitin-Binding Protein, CBP21, in Serratia marcescens 2170
复制标题
LysR 型转录调节因子 ChiR 对于粘质沙雷氏菌 2170 中所有几丁质酶和几丁质结合蛋白 CBP21 的产生至关重要
DOI：
--
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Takeshi Watanabe
中科院分区：
文献类型：
--
作者：
Kazushi Suzuki;Takuma Uchiyama;Megumi Suzuki;N. Nikaidou;M. Regué;Takeshi Watanabe
为了鉴定粘质沙雷氏菌2170产生几丁质酶所需的基因，在先前的研究中分离出了几丁质酶产生中有某种缺陷的各种Tn5突变体。为了鉴定几丁质酶缺陷突变体之一N1中的突变基因，克隆并测序了Tn5插入侧翼的DNA区域。序列分析表明，突变发生在chiB和cbp之间的ORF上，分别编码几丁质酶B和几丁质结合蛋白CBP 21。该ORF编码一个313个氨基酸的多肽，与多种LysR型转录调控因子具有显著的相似性，因此该基因被命名为chiR。靶向诱变证实，在N1的表型的chiR基因的结果的破坏。使用部分纯化的ChiR蛋白的凝胶迁移率变动分析表明，该蛋白质特异性结合到ChiR和cbp之间的基因间区域。这些结果有力地表明，ChiR是一个LysR型转录调控因子，这是必不可少的生产的所有几丁质酶和CBP 21。
To identify the genes required for chitinase production by Serratia marcescens 2170, various Tn5 mutants somehow defective in chitinase production were isolated in a previous study. In order to identify the mutated gene in one of the chitinase-deficient mutants, N1, DNA regions flanking the Tn5 insertion were cloned and sequenced. Sequence comparison showed that the mutation occurred in the ORF located between chiB and cbp, which encode chitinase B and chitin-binding protein CBP21, respectively. The ORF encodes a 313-amino acid polypeptide which has significant similarity with various LysR-type transcriptional regulators, and thus the gene was designated chiR. Targeted mutagenesis confirmed that disruption of the chiR gene results in the phenotype of N1. Gel mobility shift assays using partially purified ChiR protein demonstrated that this protein specifically binds to the intergenic region between chiR and cbp. These results strongly suggest that ChiR is a LysR-type transcriptional regulator which is essential for production of all chitinases and CBP21.
DOI：
10.1016/s0021-9258(18)61070-1
发表时间：
1987-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Matsudaira
通讯作者：
P. Matsudaira