The Linear Element is a Stable Structure Along the Chromosome Axis in Fission Yeast.
The Linear Element is a Stable Structure Along the Chromosome Axis in Fission Yeast.
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线性元件是裂殖酵母中沿染色体轴的稳定结构。
DOI：
10.1101/2020.07.03.185785
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Hiraoka Y.
中科院分区：
文献类型：
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作者：
Ding DQ;Matsuda A;Okamasa K;Hiraoka Y.
进入减数分裂后，染色体结构发生巨大变化，以确保减数分裂特异性事件的成功进展。在此过程中，许多真核生物中形成了称为联会复合体（SC）的多层蛋白质结构。相反，在裂殖酵母粟酒裂殖酵母中，线性元件 (LinEs) 是与 SC 轴向元件相关的结构，在基于减数分裂粘连蛋白的染色体轴上形成，并且是形成 DNA 双链断裂所必需的。与组织良好的 SC 结构相反，LinE 结构仅通过银染电子显微照片或免疫荧光染色扩散核中观察到。因此，它们在完整活细胞中的精细结构和动力学仍有待阐明。在这项研究中，我们使用宽视场荧光显微镜和 3D 结构照明显微镜 (3D-SIM) 对 LinE 的四个组件（Rec10、Rec25、Rec27 和 Mug20）进行了活细胞成像。我们发现 LinE 由减数分裂前期沿染色体轴形成的线组成。仅当所有其他 LinE 组件存在时，Rec10 才会与染色体本身结合并形成 LinE。 Rec25、Rec27 和 Mug20 在 Rec10 存在的情况下附着在染色体上。 LinEs 在 1,6-己二醇的短时间处理下是稳定的；光漂白后的荧光恢复（FRAP）实验显示光漂白恢复缓慢，表明 LinEs 具有稳定的特性。
Chromosomes structure changes dramatically upon entering meiosis to ensure the successful progression of meiosis-specific events. During this process, a multilayer proteinaceous structure called synaptonemal complex (SC) is formed in many eukaryotes. Instead, in the fission yeastSchizosaccharomyces pombe, linear elements (LinEs), which are structures related to an axial element of SC, form on the meiotic cohesin-based chromosome axis and are required for the formation of DNA double-strand breaks. In contrast to the well-organized SC structure, LinE structure had been observed only by silver-stained electron micrographs or in immuno-fluorescence stained spread nuclei. Thus, their fine structure and dynamics in intact living cells remain to be elucidated. In this study, we performed live cell imaging with wide-field fluorescence microscopy as well as 3D structured illumination microscopy (3D-SIM) for the four components of LinE, the Rec10, Rec25, Rec27 and Mug20. We found that LinEs consist of threads formed along the chromosome axes during the meiotic prophase. Rec10 binds to the chromosome itself and shapes into LinEs only in the presence of all the other LinE components. Rec25, Rec27, and Mug20 attach to the chromosome in the presence of Rec10. LinEs are stable in a short-time treatment with 1,6-hexanediol; and fluorescence recovery after photobleaching (FRAP) experiment reveals slow recovery from photobleaching, indicating a stable property of LinEs.