Identification of cytochromes P450 1A2, 2A1, 2C7, 2E1 in rat glioma C6 cell line by RT-PCR and specific restriction enzyme digestion.
Identification of cytochromes P450 1A2, 2A1, 2C7, 2E1 in rat glioma C6 cell line by RT-PCR and specific restriction enzyme digestion.
复制标题
通过 RT-PCR 和特异性限制性酶消化鉴定大鼠胶质瘤 C6 细胞系中的细胞色素 P450 1A2、2A1、2C7、2E1。
DOI：
10.1006/bbrc.1993.2601
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发表时间：
1993
影响因子：
3.1
通讯作者：
Strobel,HW
中科院分区：
文献类型：
--
作者：
Geng,J;Strobel,HW
采用逆转录/聚合酶链式反应(RT/PCR)检测大鼠脑胶质瘤C6细胞系中细胞色素P450的5个亚家族的6种亚型的表达。RT-PCR检测到P450 1A2、2A1、207、2E1在未经处理的细胞以及经苯巴比妥(PB)或苯并(A)菲(BA)处理的细胞中均有表达。P4503A和2C11虽然在我们先前的研究中在肝组织中检测到，但在我们的胶质瘤标本中没有检测到。为了确定合适的扩增产物，用不同的限制性内切酶对扩增片段进行酶切，得到了预期的酶切图谱。这些结果首次表明，代表大鼠中枢神经系统(CNS)的单细胞类型的胶质瘤C6细胞含有P450依赖的代谢系统，这似乎对了解药物代谢、神经传递以及脑内肿瘤的病因和化疗具有重要意义。
Reverse transcription (RT)/polymerase chain reaction (PCR) was used to detect the expression of six isoforms of cytochrome P450 which belong to five P450 subfamilies in rat glioma C6 cell line. P450 1A2, 2A1, 207, 2E1 were identified by RT-PCR in all samples, including untreated cells as well as cells treated with phenobarbital (PB) or benzo(a)anthracene (BA). P450 3A and 2C11 were not detected in our glioma samples although they were detected in liver tissues in our previous study. To confirm proper PCR products, various restriction enzymes were used to digest the PCR fragments and the expected digestion patterns were obtained. These results demonstrate for the first time that glioma C6 cells, representing a single cell type of rat central nervous system (CNS), contain a P450-dependent metabolism system which seems important for understanding drug metabolism, neurotransmission as well as tumor etiology and chemotherapy in brain.