Transcriptome-wide identification and expression analysis of chrysanthemum SBP-like transcription factors
Transcriptome-wide identification and expression analysis of chrysanthemum SBP-like transcription factors
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菊花SBP样转录因子的全转录组鉴定及表达分析
DOI：
10.1016/j.plaphy.2016.02.009
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发表时间：
2016-05-01
影响因子：
6.5
通讯作者：
Chen, Fadi
中科院分区：
文献类型：
--
作者：
Song, Aiping;Gao, Tianwei;Chen, Fadi
SQUAMOSA启动子结合蛋白（SBP）转录因子在植物的许多过程中发挥作用。在这里，我们已经确定了12 SBP-like（SPL）基因在重要的观赏植物菊花（Chrysanthemum morifolium）。基于转录组序列，共分离并扩增了12个不同的序列。系统发育分析鉴定了拟南芥和菊花的两对正向同源蛋白和菊花的两对旁系同源蛋白。使用MEME扫描拟南芥和菊花共有的SPL蛋白中的保守基序。生物信息学分析显示，这些基因中有6个含有miR 156靶位点，而5个CmSPL被miR 157靶向。此外，我们使用5' RLM-RACE来定位CmSPL 2和CmSPL 3中的切割位点。这12个基因的表达响应于各种植物激素处理和非生物胁迫的特点。这项工作提高了我们对SPL基因家族成员在应激反应中的各种功能的理解。(C)2016年由Elsevier Masson SAS出版。
SQUAMOSA promoter-binding protein (SBP) transcription factors are known to function in a number of processes in plants. Here, we have characterized twelve SBP-like (SPL) genes in the important ornamental species chrysanthemum (Chrysanthemum morifolium). A total of twelve distinct sequences were isolated and amplified based on transcriptomic sequences. Phylogenetic analysis identified two pairs of orthologous proteins for Arabidopsis and chrysanthemum and two pairs of paralogous proteins in chrysanthemum. Conserved motifs in the SPL proteins shared by Arabidopsis and chrysanthemum were scanned using MEME. A bioinformatics analysis revealed that six of these genes contained a miR156 target site, while five CmSPLs were targeted by miR157. Moreover, we used 5' RLM-RACE to map the cleavage sites in CmSPL2 and CmSPL3. The expression of these twelve genes in response to a variety of phytohormone treatments and abiotic stresses was characterized. This work improves our understanding of the various functions of SPL gene family members in the stress response. (C) 2016 Published by Elsevier Masson SAS.