Application of proteomics for comparison of proteome of Neospora caninum and Toxoplasma gondii tachyzoites
Application of proteomics for comparison of proteome of Neospora caninum and Toxoplasma gondii tachyzoites
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DOI：
10.1016/j.jchromb.2004.08.048
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发表时间：
2005-02-05
影响因子：
3
通讯作者：
Jung, TS
中科院分区：
文献类型：
--
作者：
Lee, EG;Kim, JH;Jung, TS
通过蛋白质组学方法研究了两种犬新孢子虫分离株（KBA-2 和 JPA1）和弓形虫 RH 菌株的蛋白质谱。在犬新孢子虫的 KBA-2 和 JPA1 之间，二维凝胶电泳 (2-DE) 图谱上大约 78% 的蛋白质点和 2-DE 免疫印迹图谱上 80% 的抗原点具有相同的 pI 和 M。另一方面，使用针对犬新孢子虫KBA-2的兔抗血清在2-DE免疫印迹谱上总共识别出30个弓形虫抗原点。许多同源蛋白，如热休克蛋白 70、微管蛋白 α 和 β 链、推定蛋白二硫键异构酶、肌动蛋白、烯醇化酶和 14-3-3 蛋白同源物被认为是犬新孢子虫和弓形虫中的保守蛋白。相反，NcSUB1、NcGRA2 和 NCDG1 (NcGRA7) 可能是犬新孢子虫速殖子的物种特异性蛋白。本研究表明，犬新孢子虫分离株（KBA-2 和 JPA1）之间具有高度相似性，而通过蛋白质组比较发现犬新孢子虫和刚地弓形虫之间存在较大差异。 (C) 2004 Elsevier B.V. 保留所有权利。
Protein profiles of two isolates of Neospora caninum (KBA-2 and JPA1) and Toxoplasma gondii RH strain were investigated by proteomic approach. Approximately, 78% of protein spots on two-dimensional gel electrophoresis (2-DE) profiles and 80% of antigen spots on 2-DE immunoblotting profiles were exhibited to share the same pI and M, between KBA-2 and JPA1 of N. caninum. On the other hand, a total of 30 antigen spots of T gondii were recognized on 2-DE immunoblotting profile using rabbit antiserum against N. caninum KBA-2. A number of homologue proteins, such as heat shock protein 70, tubulin alpha- and beta-chain, putative protein disulfide isomerase, actin, enolase and 14-3-3 protein homologue are believed as the conserved proteins in both N. caninum and T gondii. On the contrary, NcSUB1, NcGRA2 and NCDG1 (NcGRA7) might be the species-specific proteins for N. caninum tachyzoites. The present study showed that the high degree of similarity between N. caninum isolates (KBA-2 and JPA1), whereas large differences between N. caninum and T. gondii were noticed by proteome comparisons. (C) 2004 Elsevier B.V. All rights reserved.