INVOLVEMENT OF THE MYENTERIC PLEXUS IN THE CHOLERA-TOXIN - INDUCED NET FLUID SECRETION IN THE RAT SMALL-INTESTINE
INVOLVEMENT OF THE MYENTERIC PLEXUS IN THE CHOLERA-TOXIN - INDUCED NET FLUID SECRETION IN THE RAT SMALL-INTESTINE
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DOI：
10.1016/0016-5085(93)90130-5
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发表时间：
1993-11-01
期刊：
影响因子：
29.4
通讯作者：
SJOQVIST, A
中科院分区：
文献类型：
--
作者：
JODAL, M;HOLMGREN, S;SJOQVIST, A
背景：肠神经系统在体内负责霍乱毒素引起的大多数液体转运变化。本研究的目的是调查的重要性，肌间神经丛的壁内反射负责这种分泌。方法：采用苯扎氯铵对大鼠空肠肌间神经丛进行长时程阻断。结果：无功能性肌间神经丛的治疗节段显示正常的净液体吸收。霍乱毒素在这段只引起减少液体吸收，而在未处理的回肠段，它伴随着引起明显的净液体分泌。静脉注射六烃季铵并没有改变霍乱毒素的反应，在处理的空肠段，而血管活性肠多肽引起了显着的分泌。结论：苯扎氯铵治疗消除了霍乱毒素诱导肠道分泌的能力。因此，霍乱毒素激活的肠壁内分泌反射中的所有传入纤维可能都是通过肌间神经丛传递的，肌间神经丛是肠神经系统的整合中心。当研究管腔促分泌素的作用时，不能使用剥离的肠道准备的Ussing室技术。
Background: The enteric nervous system is responsible in vivo for most of the change in fluid transport induced by cholera toxin. The aim of the present study was to investigate the importance of the myenteric plexus in the Intramural reflex responsible for this secretion. Methods: Long-term ablation of the myenteric plexus was achieved by serosal application of benzalkonium chloride on jejunal segments in rats. Results: The treated segments without functioning myenteric plexus showed a normal net fluid absorption. Cholera toxin in this segment only induced a reduction of fluid absorption, whereas in a nontreated lleal segment it concomitantly induced a conspicuous net fluid secretion. Intravenous hexamethonium did not change the cholera toxin response in the treated jejunal segments, whereas vasoactive intestinal polypeptide elicited a marked secretion. Conclusions: Benzalkonlum chloride treatment eliminated the ability of cholera toxin to induce intestinal secretion. Thus, all afferent fibers in the intramural secretory reflex activated by cholera toxin are probably conveyed via the myenteric plexus, which functions as the integrating center in the enteric nervous system. The Ussing chamber technique using stripped intestinal preparations cannot be used when studying effects of luminal secretagogues.