Osmotically Driven and Detected DNA Translocations
Osmotically Driven and Detected DNA Translocations
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DOI：
10.1038/s41598-019-51049-4
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发表时间：
2019-10
影响因子：
4.6
通讯作者：
A. McMullen;George Araujo;M. Winter;D. Stein
中科院分区：
文献类型：
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作者：
A. McMullen;George Araujo;M. Winter;D. Stein
盐度梯度推动DNA分子通过固态纳米孔并产生离子电流，其变化允许检测易位。测量和理论分析揭示了扩散渗透在驱动这些现象中的作用：在考虑已知的盐度依赖性电极效应后，由纳米孔内DNA分子的存在引起的测量电流变化和通过纳米孔的DNA易位速度都随着所施加的盐度梯度的大小而增加。这些效应与扩散渗透理论一致，并且足够强，使得DNA易位能够克服数十毫伏的外加阻滞电位。这项工作说明了盐度梯度如何用于为纳米孔传感器供电和操作。
A salinity gradient propels a DNA molecule through a solid-state nanopore and generates an ionic current whose change allows for the detection of the translocation. Measurements and theoretical analyses reveal the role of diffusio-osmosis in driving these phenomena: After accounting for known salinity-dependent electrode effects, the measured current change caused by the presence of a DNA molecule inside the nanopore and the DNA translocation speed through it both increase with the magnitude of the applied salinity gradients. The effects are consistent with the theory of diffuisio-osmosis and strong enough to enable DNA translocations to overcome an applied retarding potential of tens of millivolts. This work illustrates how salinity gradients can be used to power and operate a nanopore sensor.