The role of insulator elements in defining domains of gene expression
The role of insulator elements in defining domains of gene expression
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DOI：
10.1016/s0959-437x(97)80134-7
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发表时间：
1997-04-01
影响因子：
4
通讯作者：
Geyer, PK
中科院分区：
文献类型：
--
作者：
Geyer, PK
绝缘子是天然存在的DNA序列，可以保护转基因免受基因组位置效应的影响，从而在染色体内建立独立的功能结构域。最近的研究集中在识别绝缘子活性的顺式和反式要求上。这些实验表明，绝缘子中含有多个组分，它们协同作用赋予绝缘子独特的性能，此外，这些实验还表明，绝缘子的绝缘机理与增强功能有关。绝缘体功能可以考虑两种模型：结构域边界和转录诱饵模型。
Insulators are naturally occurring DNA sequences that protect transgenes from genomic position effects, thereby establishing independent functional domains within the chromosome. Recent studies have focused on the identification of the cis and trans requirements for insulator activity. These experiments demonstrate that insulators contain multiple components that cooperate to confer their unique properties, Additionally, they suggest that the mechanism of insulation is related to that of enhancer function. Two models of insulator function can be considered: a domain boundary and a transcriptional decoy model.