Cytoplasmic-only Expression of Maspin Predicts Poor Prognosis in Patients With Oral Squamous Cell Carcinoma
Cytoplasmic-only Expression of Maspin Predicts Poor Prognosis in Patients With Oral Squamous Cell Carcinoma
复制标题
DOI：
10.21873/anticanres.15269
复制
发表时间：
2021-09-01
影响因子：
2
通讯作者：
Umekita, Yoshihisa
中科院分区：
文献类型：
--
作者：
Kawasaki, Makoto;Sakabe, Tomohiko;Umekita, Yoshihisa
背景/目的：Maspin具有肿瘤抑制作用，但其对口腔鳞状细胞癌(OSCC)患者的预后价值尚不清楚。我们的目的是评估口腔鳞癌患者中maspin亚细胞定位对预后的重要性。患者和方法：对80例手术切除标本进行免疫组织化学分析。仅在细胞质中观察到的10%的肿瘤被定义为maspin阳性。结果：maspin阳性状态(25%)与较高的复发率和较短的无病生存期(DFS)显著相关。Cox‘s多变量分析显示，maspin阳性状态是缩短DFS的独立因素。免疫印迹分析显示所有口腔鳞癌细胞系(HSC2、HSC3、HSC4、Ca9-22和SAS)的maspin蛋白均定位于胞浆和胞核。在HSC4细胞中，maspin基因被敲除后，细胞侵袭力显著增加。结论：仅胞浆maspin表达可能是口腔鳞癌患者预后不良的独立因素。
Background/Aim: Maspin has tumor-suppressor functions; however, its prognostic value in patients with oral squamous cell carcinoma (OSCC) remains unknown. We aimed to assess the prognostic importance of the subcellular localization of maspin in patients with OSCC. Patients and Methods: Eighty resected specimens were analyzed by immunohistochemistry. Cytoplasmic-only expression observed in >10% of the tumor was defined as maspin-positive. Results: The maspin-positive status (25%) was significantly associated with a higher recurrence rate and shorter disease-free survival (DFS). Cox's multivariate analysis showed that maspin-positive status was an independent factor for shorter DFS. All OSCC cell lines (HSC2, HSC3, HSC4, Ca9-22 and SAS) showed maspin protein localization to both the cytoplasm and nucleus using western blot analysis. In HSC4 cells, cell invasion was significantly increased in response to maspin knockdown. Conclusion: Cytoplasmic-only expression of maspin could be an independent poor prognostic factor for patients with OSCC.