Quantitative histological analysis and ultrastructure of the aging human testis
Quantitative histological analysis and ultrastructure of the aging human testis
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DOI：
10.1007/s11255-013-0610-0
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发表时间：
2014-05-01
影响因子：
2
通讯作者：
Gu, Yi-Qun
中科院分区：
文献类型：
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作者：
Jiang, Huan;Zhu, Wei-Jie;Gu, Yi-Qun
目的定量评价人类睾丸衰老引起的组织学和超微结构变化。采用光镜、透射电镜（TEM）和免疫组化技术对41例老年男性睾丸样本进行年龄相关性组织学和超微结构变化评估，这些样本分别被分为A组（n = 20）、54-69岁组和B组（n = 21）、70-89岁组。来自17名年轻男性的睾丸样本作为对照。老龄组的支持细胞数量显著低于对照组（p < 0.05）。除精原细胞和原代精母细胞的支持细胞比率（生殖细胞/支持细胞）外，老年男性圆形和细长精子细胞的支持细胞比率低于年轻男性（p < 0.05）。在透射电镜下，衰老睾丸的生殖细胞和支持细胞也显示出类似的细胞器退化模式。免疫组化结果显示，B组细胞凋亡指数（AI）升高（0.81 +/- A 0.13），增殖指数（PI）降低（30.08 +/- A 4.86） （p < 0.05），而A组（0.54 +/- A 0.06; 36.38 +/- A 7.38）和对照组（0.50 +/- A 0.15; 40.55 +/- A 7.92）的AI和PI基本相同（p < 0.05）。衰老对睾丸形态和精子发生有负面影响，从精子水平上可以明显看出生精细胞发育的失败。
To quantitatively assess the histological and ultrastructural changes resulting from aging in the human testis.Age-related histological and ultrastructural changes were evaluated using light microscopy, transmission electron microscopy (TEM) and immunohistochemistry on 41 testicular samples obtained from elderly men and, respectively, assigned to group A (n = 20), 54-69 years old or group B (n = 21), 70-89 years old. Testicular samples derived from 17 young men were used for control.The numbers of Sertoli cells in the aged groups were significantly lower than that in the controls (p < 0.05). With the exception of the Sertoli cell ratios (germ cells/Sertoli cells) of spermatogonia and primary spermatocytes, results showed lower levels of the Sertoli cell ratios of round spermatids and elongated spermatids in the elderly men compared with the young men (p < 0.05). A similar degenerative pattern of the organelles was shown in germ cells and Sertoli cells in the aging testes under TEM. Immunohistochemistry revealed an increased apoptosis index (AI) (0.81 +/- A 0.13) accompanied by a decreased proliferation index (PI) (30.08 +/- A 4.86) in the group B (p < 0.05), while both AI and PI were similar between the group A (0.54 +/- A 0.06; 36.38 +/- A 7.38) and the controls (0.50 +/- A 0.15; 40.55 +/- A 7.92) (p > 0.05).Aging has negative influence on testicular morphology and spermatogenesis, and the failure of spermatogenic cell development is evident from the spermatid level.