The effects of starvation and temperature acclimation on pentose phosphate pathway dehydrogenases in brook trout liver.
The effects of starvation and temperature acclimation on pentose phosphate pathway dehydrogenases in brook trout liver.
复制标题
饥饿和温度驯化对溪鳟鱼肝脏中磷酸戊糖途径脱氢酶的影响。
DOI：
10.1016/0003-9861(75)90436-1
复制
发表时间：
1975
影响因子：
3.9
通讯作者：
E. Goldberg
中科院分区：
文献类型：
--
作者：
T. Yamauchi;J. Stegeman;E. Goldberg
温度和饥饿是影响溪鳟肝脏PPP脱氢酶活性的主要因素。在5 °C下驯化的鱼比在10或15 °C下驯化的鱼具有更高水平的G6PD、H6PD和6PGD活性。这一现象可能与冷驯化过程中脂肪生成增加有关，饥饿时肝脏G6PD和6PGD活性下降，而H6PD活性略有增加。在再喂养，G6PD水平逐渐增加，但在哺乳动物的研究中报告的酶活性的“过冲”没有observed.When冷驯化和饥饿同时进行了研究，温度调节最初是占主导地位的控制因素。在5 °C下6周后，饥饿和喂养的鱼之间的比活性没有差异。然而，在5 °C下保持超过2个月的鱼确实表现出对饥饿和再喂食的正常反应。因此，温度对PPP的调节似乎是一种暂时的现象，可能与鱼的暂时代谢重组有关。
Temperature and starvation were found to be factors which affected the PPP dehydrogenase activities in brook trout liver. Fish acclimated at 5 °C possessed greater levels of G6PD, H6PD, and 6PGD activity than those fish maintained at 10 or 15 °C. This phenomenon was probably associated with increased lipogenesis during cold acclimation.During starvation hepatic G6PD and 6PGD activities decreased, whereas H6PD activity increased slightly. Upon refeeding, the G6PD level gradually increased, but the “overshoot” in enzyme activity reported in mammalian studies was not observed.When both cold acclimation and starvation were studied simultaneously, regulation by temperature was initially the dominant control factor. After 6 wk at 5 °C, there was no difference in specific activities between starved and fed fish. However, fish maintained at 5 °C for longer than 2 mo did show the normal response to starvation and refeeding. Therefore, regulation of the PPP by temperature appears to be a transitory phenomenon and may be associated with temporary metabolic reorganization in the fish.