Microcracks in compact bone: a three-dimensional view
Microcracks in compact bone: a three-dimensional view
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DOI：
10.1111/j.1469-7580.2006.00554.x
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发表时间：
2006-07-01
影响因子：
2.4
通讯作者：
Lee, T. C.
中科院分区：
文献类型：
--
作者：
Mohsin, S.;O'Brien, F. J.;Lee, T. C.
微裂纹与骨质疏松症的骨质量损失有关。为了检测和监测它们的生长，并使用这些数据来预测裂缝，必须获得关于它们在三维中的形状的定量数据。制备羊桡骨梁状骨样品，用荧光染料染色，并在伺服液压疲劳试验机上进行四点弯曲下的循环疲劳试验。在100 MPa的应力范围下，在负载控制下以30 Hz的频率对样本进行测试。在骨样本中钻孔并用作重建的参考点。使用滑橇式大切片机切割一系列薄的纵向切片。使用落射荧光显微镜检查每个切片的二维图像，并通过CCD低光彩色摄像机将图像传输到PC。使用计算机软件重建每个微裂纹的三维图像，并测量其尺寸。裂纹呈椭圆形，纵向较长，平均纵横比为5.5 +/- 1.05。标记微裂纹的平均（+/- SD）长度和宽度分别为488 +/- 151和88 +/- 21 μ m。
Microcracks have been implicated in the loss of bone quality for osteoporosis. In order to detect and monitor their growth, and to use these data to predict fractures, it is essential to obtain quantitative data regarding their shape in three dimensions. Beam-shaped bone samples from sheep radii were prepared and stained with fluorochrome dyes and tested in cyclical fatigue under four-point bending in a servo-hydraulic fatigue-testing machine. Samples were tested at a frequency of 30 Hz under load control at a stress range of 100 MPa. Holes were drilled into bone samples and used as reference points for reconstructions. A series of thin longitudinal sections were cut using a sledge macrotome. A two-dimensional image of each section was examined using an epifluorescence microscope and images transferred to a PC via a CCD low-light colour video camera. A three-dimensional image of each microcrack was reconstructed using computer software, and its dimensions measured. Cracks were elliptical in shape, longer in the longitudinal direction and with a mean aspect ratio of 5.5 +/- 1.05. The mean (+/- SD) length and width of labelled microcracks were 488 +/- 151 and 88 +/- 21 mu m, respectively.