Production of sophorolipids from winterization oil cake by solid-state fermentation: Optimization, monitoring and effect of mixing
Production of sophorolipids from winterization oil cake by solid-state fermentation: Optimization, monitoring and effect of mixing
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DOI：
10.1016/j.bej.2016.08.006
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发表时间：
2016-11-15
影响因子：
3.9
通讯作者：
Font, Xavier
中科院分区：
文献类型：
--
作者：
Jimenez-Penalver, Pedro;Gea, Teresa;Font, Xavier
槐糖脂 (SL) 是一组由 Starmerellaombicola 酵母产生的细胞外生物表面活性剂。本研究探索了使用冬化油饼（WOC）（一种来自炼油行业的残余油饼）作为固态发酵（SSF）生产SL的底物。甜菜糖蜜 (MOL) 用作辅助底物，S. bidicola ATCC 22214 用作接种物。发酵以 100 克规模进行，并使用响应面法对底物比例和通气速率进行优化。优化的 SSF 工艺（1:4 MOL:WOC 质量比和 0.30 L kg(-1) min(-1) 通气速率）在静态条件下进行，监测 10 天，最大 SL 产量为 0.179 g/g DM（干物质）。还研究了间歇混合对过程的影响。混合使 SL 产量增加 31%，总产量为每克 DM 0.235 克。摄氧率 (OUR) 和累积耗氧量 (COC) 用于监测发酵过程的生物活性。 SL 产量与消耗的氧气和脂肪之间存在显着相关性。通过 FTIR 和 H-1 NMR 分析对 SL 进行了表征。 (C) 2016 Elsevier B.V. 保留所有权利。
Sophorolipids (SLs) are a group of extracellular biosurfactants produced by the yeast Starmerella bombicola. The present study explored the use of winterization oil cake (WOC), a residual oil cake that comes from the oil refining industry, as a substrate for the production of SLs by solid-state fermentation (SSF). Sugar beet molasses (MOL) was used as a co-substrate and S. bombicola ATCC 22214 as the inoculum. Fermentation was performed on the 100-g scale and was optimized in terms of the ratio of substrates and the aeration rate using response surface methodology. The optimized SSF process (1:4 MOL:WOC mass ratio and 0.30 L kg(-1) min(-1) aeration rate), carried out under static conditions, was monitored for 10 days with a maximum SL yield of 0.179 g per g DM (dry matter). The effect of intermittent mixing on the process was also investigated. Mixing caused a 31% increase in SL production, with a total yield of 0.235 g per g DM. The Oxygen Uptake Rate (OUR) and the Cumulative Oxygen Consumption (COC) were used to monitor the biological activity of the fermentation processes. There were significant correlations between the SL yield and the oxygen and fats consumed. The SLs were characterized by FTIR and H-1 NMR analysis. (C) 2016 Elsevier B.V. All rights reserved.