Label-free and selective sensing of uric acid with gold nanoclusters as optical probe
Label-free and selective sensing of uric acid with gold nanoclusters as optical probe
复制标题
以金纳米簇作为光学探针对尿酸进行无标记和选择性传感
DOI：
10.1016/j.talanta.2016.01.018
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发表时间：
2016
期刊：
影响因子：
6.1
通讯作者：
Huang Cheng Zhi
中科院分区：
文献类型：
--
作者：
Wang Jian;Chang Yong;Wu Wen Bi;Zhang Pu;Lie Shao Qing;Huang Cheng Zhi
Clinically, the amount of uric acid (UA) in biological fluids is closely related to some diseases such as hyperuricemia and gout, thus it is of great significance to sense UA in clinical samples. In this work, red gold nanoclusters (AuNCs) with relatively high fluorescence quantum yield and strong fluorescence emission were facilely available using bovine serum albumin (BSA) as template. The fluorescence of BSA-protected AuNCs can be sensitively quenched by H2O2, which is further capable of sensing UA through the specific catalytic oxidation with uricase, since it generates stoichiometric quantity of H2O2by-product. The proposed assay allows for the selective detection of UA in the range of 10–800 μM with a detection limit of 6.6 μM, which is applicable to sense UA in clinical samples with satisfactory results, suggesting its great potential for diagnostic purposes.
