The plasticizer butyl benzyl phthalate induces genomic changes in rat mammary gland after neonatal/prepubertal exposure.
The plasticizer butyl benzyl phthalate induces genomic changes in rat mammary gland after neonatal/prepubertal exposure.
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DOI：
10.1186/1471-2164-8-453
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发表时间：
2007-12-06
期刊：
影响因子：
4.4
通讯作者：
Russo, Jose
中科院分区：
文献类型：
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作者：
Moral, Raquel;Wang, Richard;Russo, Irma H;Mailo, Daniel A;Lamartiniere, Coral A;Russo, Jose
邻苯二甲酸酯如邻苯二甲酸正丁基苄基酯（BBP）是广泛使用的增塑剂。BBP已显示出内分泌干扰特性，因此对乳腺癌敏感组织具有潜在影响。本研究的目的是确定新生儿/青春期前（出生后2-20天）暴露于BBP对不同发育年龄（21、35、50和100天）大鼠乳腺成熟参数和形态学、增殖指数和基因组特征的影响。在这里，我们表明，暴露于BBP增加子宫重量/体重比在21天，并降低阴道开口时的体重。BBP没有引起乳腺形态的显着变化，但增加了增殖指数在35天的终端芽和小叶1在几个年龄。此外，BBP主要在暴露结束时（21天）对乳腺的基因组图谱产生影响，此后变得不那么突出。到这个年龄，大量与增殖和分化、通讯和信号转导相关的基因在暴露动物的腺体中上调。这些结果表明BBP对乳腺的基因表达谱有影响。
Phthalate esters like n-butyl benzyl phthalate (BBP) are widely used plasticizers. BBP has shown endocrine-disrupting properties, thus having a potential effect on hormone-sensitive tissues. The aim of this study is to determine the effect of neonatal/prepubertal exposure (post-natal days 2–20) to BBP on maturation parameters and on the morphology, proliferative index and genomic signature of the rat mammary gland at different ages of development (21, 35, 50 and 100 days). Here we show that exposure to BBP increased the uterine weight/body weight ratio at 21 days and decreased the body weight at time of vaginal opening. BBP did not induce significant changes on the morphology of the mammary gland, but increased proliferative index in terminal end buds at 35 days and in lobules 1 at several ages. Moreover, BBP had an effect on the genomic profile of the mammary gland mainly at the end of the exposure (21 days), becoming less prominent thereafter. By this age a significant number of genes related to proliferation and differentiation, communication and signal transduction were up-regulated in the glands of the exposed animals. These results suggest that BBP has an effect in the gene expression profile of the mammary gland.