Neuromuscular junction changes in aged rat thyroarytenoid muscle
Neuromuscular junction changes in aged rat thyroarytenoid muscle
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DOI：
10.1177/000348940211100703
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发表时间：
2002-07-01
影响因子：
1.4
通讯作者：
Heisey, DM
中科院分区：
文献类型：
--
作者：
Connor, NP;Suzuki, T;Heisey, DM
神经肌肉接头(NMJ)结构的动态重构被认为是年龄相关性肌萎缩的原因。直接研究喉肌肉中NMJ的形态对于我们理解与年龄相关的发声和吞咽功能减退是很重要的。为了比较年轻和老年大鼠甲颧肌(一种对呼吸道保护和发声至关重要的肌肉)的形态，对NMJ的形态进行了研究，并使用荧光、三标记免疫组织化学分析和共聚焦显微镜可视化来分析NMJ的结构。我们发现，喉NMJ经历了与去神经支配后相似的显著变化。具体地说，轴突终末面积显著减少，有许多突触后乙酰胆碱受体区域未被神经末梢占据，旧肌终板结构的可变性增加。这项研究的结果增加了我们对喉部与年龄相关的形态变化的了解，并可能作为测试未来干预措施有效性的基线。
Dynamic remodeling of neuromuscular junction (NMJ) structure is postulated as a cause of age-related muscular atrophy. Direct study of NMJ morphology in laryngeal muscles is important to our understanding of age-related decrements in voice and swallowing. The morphology of NMJs was studied in a rat model to compare young and old specimens of thyroarytenoid muscle - a muscle critical to airway protection and phonation, Fluorescent, triple-label immunohistochemical analysis and confocal microscopic visualization were used to analyze the structure of NMJs. We found that laryngeal NMJs underwent significant changes that were similar to those observed after denervation. Specifically, the axon terminal area was significantly reduced, there were a number of postsynaptic acetylcholine receptor areas unoccupied by nerve terminals, and there was increased variability in end plate architecture in the old muscles. The results of this study increase our understanding of the age-related morphological changes in the larynx, and may serve as a baseline to test the effectiveness of future interventions.