Importance of tyrosine in the RNA-binding domain of human parainfluenza virus type 2 nucleoprotein for polymerase activity
Importance of tyrosine in the RNA-binding domain of human parainfluenza virus type 2 nucleoprotein for polymerase activity
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人副流感病毒 2 型核蛋白 RNA 结合域中酪氨酸对聚合酶活性的重要性
DOI：
10.1007/s00705-019-04240-x
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发表时间：
2019
影响因子：
2.7
通讯作者：
Nishio Machiko
中科院分区：
文献类型：
--
作者：
Matsumoto Yusuke;Ohta Keisuke;Nishio Machiko
人副流感病毒2型（hPIV 2）的RNA基因组被核蛋白（NP）包裹，作为RNA合成的模板。我们使用微型复制子测定法检查了hPIV2 NP RNA结合结构域中单个氨基酸对聚合酶活性的重要性。我们发现，位于NP的RNA结合口袋中的氨基酸位置260处的酪氨酸取代严重降低了聚合酶活性。Y260的芳香族侧链可能是核苷酸和碱性氨基酸之间形成稳定接触所必需的，从而影响病毒聚合酶对启动子的识别。
The RNA genome of human parainfluenza virus type 2 (hPIV2) is encapsidated by nucleoprotein (NP) to act as a template for RNA synthesis. We examined the importance of individual amino acids in the RNA-binding domain of hPIV2 NP for polymerase activity using a mini-replicon assay. We showed that substitution of tyrosine at amino acid position 260, located in the RNA-binding pocket of NP, severely reduced polymerase activity. The aromatic side-chain of Y260 may be required for the formation of stable contacts between nucleotides and basic amino acids, thereby affecting promoter recognition by the viral polymerase.