STRUCTURE OF INTRACELLULAR MATURE VACCINIA VIRUS OBSERVED BY CRYOELECTRON MICROSCOPY
STRUCTURE OF INTRACELLULAR MATURE VACCINIA VIRUS OBSERVED BY CRYOELECTRON MICROSCOPY
复制标题
DOI：
10.1128/jvi.68.3.1935-1941.1994
复制
发表时间：
1994-03-01
影响因子：
5.4
通讯作者：
WITTEK, R
中科院分区：
文献类型：
--
作者：
DUBOCHET, J;ADRIAN, M;WITTEK, R
细胞内成熟痘苗病毒，也称为细胞内裸露病毒，及其核心包膜已通过玻璃化样品的冷冻电镜观察到其天然的、未固定的、未染色的、水合的状态。病毒颗粒看起来像一个光滑的圆角矩形，直径约 100 毫米。 350 x 270 纳米。核心看起来是均匀的，并被由膜界定的 30 nm 厚的表面域包围。我们表明，通常在负染色或常规嵌入样品中观察到的表面小管以及很可能具有侧体的特征性哑铃形核心都是制备伪影。
Intracellular mature vaccinia virus, also called intracellular naked virus, and its core envelope have been observed in their native, unfixed, unstained, hydrated states by cryoelectron microscopy of vitrified samples. The virion appears as a smooth rounded rectangle of ca. 350 by 270 nm. The core seems homogeneous and is surrounded by a 30-nm-thick surface domain delimited by membranes. We show that surface tubules and most likely also the characteristic dumbbell-shaped core with the lateral bodies which are generally observed in negatively stained or conventionally embedded samples are preparation artifacts.