Hox complex analysis through BAC recombineering.
Hox complex analysis through BAC recombineering.
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通过 BAC 重组工程进行 Hox 复合物分析。
DOI：
10.1007/978-1-4939-1242-1_5
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
Krumlauf,Robb
中科院分区：
文献类型：
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作者：
Parrish,Mark;Ahn,Youngwook;Nolte,Christof;DeKumar,Bony;Krumlauf,Robb
BAC转基因小鼠已被证明是有用的，在探索的Hox复合物的调节机制和功能。所使用的大型结构可能包括大多数的相关组成部分的is-regulatorylandscape。操作可以在不损害内源性复合物的完整性的情况下完成，这降低了产生混淆表型异常的可能性。重组工程工具的开发在提供对这些大构建体进行多种类型的精确和不同操作所必需的手段方面是至关重要的。在这里，我们将讨论必要的方法来操纵Hox复合体BAC，产生转基因动物轴承这些结构和利用这些资源来解决的基本方面ofHox生物学。
BAC transgenesis in mice has proved to be useful in exploring the regulatory mechanisms and functions of theHoxcomplexes. The large constructs used may include most of the relevant components of thecis-regulatorylandscape. Manipulations can be accomplished without compromising the integrity of the endogenous complex which reduces the likelihood of producing confounding phenotypic abnormalities. The development of recombineering tools has been critical in providing the means necessary to make many types of precise and varied manipulations of these large constructs. Here, we will discuss the methodologies necessary to manipulateHoxcomplex BACs, generation of transgenic animals bearing these constructs and the utilization of these resources to address fundamental aspects ofHoxbiology.