The Rhodospirillum rubrum cytochrome bc1 complex: peptide composition, prosthetic group content and quinone binding.
The Rhodospirillum rubrum cytochrome bc1 complex: peptide composition, prosthetic group content and quinone binding.
复制标题
红色红螺菌细胞色素 bc1 复合物：肽组成、辅基含量和醌结合。
DOI：
10.1016/s0005-2728(89)80190-2
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Knaff,DB
中科院分区：
文献类型：
--
作者：
Kriauciunas,A;Yu,L;Yu,CA;Wynn,RM;Knaff,DB
细胞色素bc1复合物基本上不含细菌叶绿素，已从光合紫色非硫细菌红色红螺菌中纯化出来。该复合物催化从对苯二酚到细胞色素的电子流（周转数 = 75s−1），该电子流被低浓度的抗霉素 A 和 myxothiazol 抑制。该复合物仅包含三个肽亚基：细胞色素b(Mr= 35000)；细胞色素c1（Mr= 31000）和Rieske铁硫蛋白（Mr= 22400）。测量细胞色素c（+ 320 mV）和细胞色素b的两个血红素（− 33和−90 mV）的Em值（pH 7.4）。当复合物在泛醌光亲和类似物 (叠氮基-Q) 存在下预照射时，从对苯二酚到细胞色素顺式的电子流受到抑制。在光照过程中，叠氮基-Q 与细胞色素肽共价连接，并在较小程度上与细胞色素 c1 共价连接。
A cytochromebc1complex, essentially free of bacteriochlorophyll, has been purified from the photosynthetic purple non-sulfur bacteriumRhodospirillum rubrum. The complex catalyzes electron flow from quinol to cytochromec(turnover number = 75s−1)that is inhibited by low concentrations of antimycin A and myxothiazol. The complex contains only three peptide subunits: cytochromeb(Mr= 35000); cytochromec1(Mr= 31000) and the Rieske iron-sulfur protein (Mr= 22400).Emvalues (pH 7.4) were measured for cytochrome c, (+ 320 mV) and the two hemes of cytochromeb(− 33 and −90 mV). Electron flow from quinol to cytochromecis inhibited when the complex is pre-illuminated in the presence of a ubiquinone photoaffinity analog (azido-Q). During illumination, the azido-Q becomes covalently attached to the cytochromebpeptide and, to a lesser extent, to cytochromec1.