ON IDENTIFYING INFORMATION FROM IMAGE-BASED SPATIAL POLARITY PHENOTYPES IN NEUTROPHILS.
ON IDENTIFYING INFORMATION FROM IMAGE-BASED SPATIAL POLARITY PHENOTYPES IN NEUTROPHILS.
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DOI：
10.1109/isbi.2010.5490165
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发表时间：
2010-04-01
期刊：
影响因子：
--
通讯作者：
Wu LF
中科院分区：
文献类型：
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作者：
Ku CJ;Wang Y;Pavie B;Altschuler SJ;Wu LF
细胞极性参与许多生物功能，如发育，伤口愈合和免疫反应。在人类嗜中性粒细胞中，极化的特征在于不同的信号分子组易位到细胞的相对末端，以及细胞骨架的快速重排以启动迁移。虽然许多基于图像的研究已经描述了细胞形态和极性信号分子的强度水平，但缺乏极性信号分子空间分布的系统表征。在这里，我们设计了一系列的分析功能，以量化极性分子的空间表型。我们将我们的功能与常用的极性读数进行了比较，发现它们捕获了现有功能中不包含的偏振动态的其他方面。我们的工作提供了一个起点，以确定信息功能的研究中性粒细胞极化。
Cell polarity is involved in many biological functions such as development, wound healing and immune responses. In human neutrophils, polarization is characterized by the translocation of distinct sets of signaling molecules to opposite ends of the cell and the rapid rearrangement of cytoskeleton to initiate migration. While many image-based studies have described cellular morphology and the intensity level of polarity signaling molecules, systematic characterization of the spatial distribution of polarity signaling molecules has been lacking. Here we designed a collection of analytical features to quantify spatial phenotypes of polarity molecules. We compared our features to commonly used polarity readouts and found that they captured additional aspects of the polarization dynamics that were not contained in the existing features. Our work provides a starting point to identify informative features for the study of neutrophil polarization.