Differential expression of the mouse cholecystokinin gene during brain and gut development.
Differential expression of the mouse cholecystokinin gene during brain and gut development.
复制标题
小鼠胆囊收缩素基因在大脑和肠道发育过程中的差异表达。
DOI：
10.1073/pnas.82.17.5593
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发表时间：
1985
影响因子：
11.1
通讯作者：
Powell,D
中科院分区：
文献类型：
--
作者：
Friedman,J;Schneider,BS;Powell,D
胆囊收缩素（CCK）是一种存在于脑和肠中的神经肽。在本报告中，我们从小鼠脑cDNA文库中分离了一个编码CCK的cDNA克隆。该cDNA克隆与先前已测序的CCK前体具有广泛的同源性。用该cDNA克隆探测的基因组DNA的Southern印迹显示了8种不同限制性内切酶中的每一种的单一条带，所有这些都可以由单个基因组克隆来解释，这表明CCK基因在小鼠中以单拷贝基因存在。RNA印迹、引物延伸和S1核酸酶保护测定表明，脑和肠中使用相同的RNA起始位点。最后，我们已经表明，通过使用RNA印迹和特异性CCK的放射免疫测定，CCK在出生时在肠中表达最高成人水平，但成人浓度的CCK及其mRNA在大脑中直到很晚才达到发展。
Cholecystokinin (CCK) is a neuropeptide found in brain and intestine. In this report, we have isolated a cDNA clone that encodes CCK from a mouse brain cDNA library. This cDNA clone has extensive homology to CCK precursors that have been sequenced previously. Southern blots of genomic DNA probed with this cDNA clone revealed single bands for each of eight different restriction enzymes, all of which could be accounted for by a single genomic clone, suggesting that the CCK gene is present as a single-copy gene in mice. RNA blots, primer extensions, and S1 nuclease protection assays have suggested that the same RNA start site is utilized in brain and in gut. Finally, we have shown, by using RNA blots and a radioimmunoassay specific for CCK, that CCK is expressed at maximum adult levels in intestine at birth but that adult concentrations of CCK and its mRNA are not reached in brain until much later in development.