A fluorescent ratiometric Cu2+ probe based on FRET by naphthalimide-appended rhodamine derivatives
A fluorescent ratiometric Cu2+ probe based on FRET by naphthalimide-appended rhodamine derivatives
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基于萘酰亚胺附加罗丹明衍生物 FRET 的荧光比率 Cu2 探针
DOI：
10.1039/c4ay01863j
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发表时间：
2014
影响因子：
3.1
通讯作者：
Zhang Jun
中科院分区：
文献类型：
--
作者：
Yu Chunwei;Wen Yingying;Qin Xi;Zhang Jun
以罗丹明B和萘二甲酰亚胺为荧光单元，通过CN键合，构建了一个荧光二联体（L），作为Cu ~（2+）的比率探针。探针L对Cu 2+的选择性荧光响应是由Cu 2+诱导罗丹明B螺环内酰胺的开环过程触发的，该开环过程基于萘酰亚胺与罗丹明B的分子内荧光共振能量转移（FRET）机制。与其他测试的金属离子和阴离子相比，该系统表现出对Cu 2+的高选择性，并且通过在580 nm处发生的罗丹明发色团的FRET介导的发射来发出结合事件的信号，荧光强度增强，同时，萘酰亚胺供体在525 nm附近的特征发射几乎完全淬灭。此外，它已被用于比率成像的Cu 2+在活细胞中与令人满意的结果。
A fluorophore dyad (L) containing rhodamine B and naphthalimide units linked through CN was constructed as a ratiometric probe for Cu2+. The selective fluorescent response of the probe L to Cu2+ was triggered by a Cu2+ induced ring-opening process of the rhodamine B spirolactam, which was based on an intramolecular fluorescence resonance energy transfer (FRET) mechanism from naphthalimide to rhodamine B. The system exhibited high selectivity for Cu2+ as compared to other tested metal ions and anions, and signaled the binding event through occurrence of FRET mediated emission at 580 nm of the rhodamine chromophore with enhancement in the fluorescence intensity, concurrently, the featured emission of the naphthalimide donor at around 525 nm was nearly completely quenched. Furthermore, it has been used for ratiometric imaging of Cu2+ in living cells with satisfying results.