Cell fibers promote proliferation of co-cultured cells on a dish
Cell fibers promote proliferation of co-cultured cells on a dish
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DOI：
10.1038/s41598-019-57213-0
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发表时间：
2020-01-14
影响因子：
4.6
通讯作者：
Takeuchi, Shoji
中科院分区：
文献类型：
--
作者：
Shima, Ai;Itou, Akane;Takeuchi, Shoji
本文描述了一种利用细胞纤维技术的共培养方法。细胞纤维是载有细胞的水凝胶微纤维，其中细胞被三维培养，并允许达到比常规二维细胞培养更成熟的状态。细胞纤维中的细胞被藻酸盐壳包裹。只有细胞分泌物通过壳释放到周围环境中，而细胞被纤维保留。由于细胞纤维具有高度的可重复性和可回收性，我们建议使用细胞纤维进行共培养，以确保细胞分泌物组的稳定供应。我们将小鼠C2C12成肌细胞与包封在细胞纤维中的小鼠3T3成纤维细胞培养两天。C2C12细胞的数量与共培养的3T3纤维的数量成比例地增加，表明3T3纤维的分泌组促进C2C12细胞的存活和增殖。我们相信，细胞纤维技术是一个有用的工具，共培养细胞，它将有助于基础细胞生物学和组织工程以其独特的功能。
This paper describes a co-culture method using cell fiber technology. Cell fibers are cell-laden hydrogel microfibers, in which cells are cultured three-dimensionally and allowed to reach more mature state than the conventional two-dimensional cell culture. Cells in the cell fibers are encapsulated by alginate shell. Only cellular secretome is released into the surrounding environment through the shell while the cells were retained by the fiber. With their high handleability and retrievability, we propose to use the cell fibers for co-culture to ensure steady supply of cellular secretome. We cultured mouse C2C12 myoblasts with mouse 3T3 fibroblasts encapsulated in the cell fibers for two days. The number of C2C12 cells increased proportionally to the number of co-cultured 3T3 fibers, suggesting that the secretome of 3T3 fibers promoted survival and proliferation of C2C12 cells. We believe that cell fiber technology is a useful tool for co-culturing cells, and it will contribute to both basic cell biology and tissue engineering with its unique features.