Relation between the fatty acid composition of peripheral blood mononuclear cells and measures of immune cell function in health free-living subjects aged 25-72 y
Relation between the fatty acid composition of peripheral blood mononuclear cells and measures of immune cell function in health free-living subjects aged 25-72 y
复制标题
DOI：
10.1093/ajcn/77.5.1278
复制
发表时间：
2003-05-01
影响因子：
7.1
通讯作者：
Calder, PC
中科院分区：
文献类型：
--
作者：
Kew, S;Banerjee, T;Calder, PC
背景资料：关于人免疫细胞的脂肪酸组成与这些细胞在脂肪酸摄入习惯范围内的功能之间的关系的信息很少。目的：本研究的目的是确定人外周血单核细胞（PBMC）磷脂的脂肪酸组成与人免疫细胞的功能之间的关系。设计：150名健康成人受试者提供了空腹血样。在全血中测定单核细胞和中性粒细胞的吞噬和氧化爆发活性。分离PBMC并用于测量响应于T细胞有丝分裂原伴刀豆球蛋白A的淋巴细胞增殖和响应于伴刀豆球蛋白A或细菌脂多糖的细胞因子的产生。血浆和PBMC磷脂的脂肪酸组成determined.Results：PBMC磷脂的脂肪酸组成和免疫细胞功能的广泛变化被确定的科目。PBMC磷脂中总多不饱和脂肪酸（PUFA）、总n-6和n-3 PUFA以及几种单独PUFA的比例与中性粒细胞和单核细胞的吞噬作用、中性粒细胞氧化爆发、淋巴细胞增殖和干扰素γ产生呈正相关。饱和脂肪酸与多不饱和脂肪酸的比例以及n-6与n-3多不饱和脂肪酸的比例与这些相同的免疫功能呈负相关。PBMC的脂肪酸组成的关系，单核细胞的氧化burst是相反的单核细胞吞噬作用和中性粒细胞的氧化burst.Conclusion：PBMC磷脂的脂肪酸组成的变化占一些健康成人之间的免疫细胞功能的变异。
Background: There is little information about the relation between the fatty acid composition of human immune cells and the function of those cells over the habitual range of fatty acid intakes.Objective: The objective of the study was to determine the relation between the fatty acid composition of human peripheral blood mononuclear cell (PBMC) phospholipids and the functions of human immune cells.Design: One hundred fifty healthy adult subjects provided a fasting blood sample. The phagocytic and oxidative burst activities of monocytes and neutrophils were measured in whole blood. PBMCs were isolated and used to measure lymphocyte proliferation in response to the T cell mitogen concanavalin A and the production of cytokines in response to concanavalin A or bacterial lipopolysaccharide. The fatty acid composition of plasma and PBMC phospholipids was determined.Results: Wide variations in fatty acid composition of PBMC phospholipids and immune cell functions were identified among the subjects. The proportions of total Polyunsaturated fatty acids (PUFAs), of total n-6 and n-3 PUFAs, and of several individual PUFAs in PBMC phospholipids were positively correlated with phagocytosis by neutrophils and monocytes, neutrophil oxidative burst, lymphocyte proliferation, and interferon gamma production. The ratios of saturated fatty acids to PUFAs and of n-6 to n-3 PUFAs were negatively correlated with these same immune functions. The relation of PBMC fatty acid composition to monocyte oxidative burst was the reverse of its relation to monocyte phagocytosis and neutrophil oxidative burst.Conclusion: Variations in the fatty acid composition of PBMC phospholipids account for some of the variability in immune cell functions among healthy adults.