Effect of γ-hydroxypropano deoxyguanosine, the major acrolein-derived adduct, on monomolecular quadruplex structure of telomeric repeat d(TTAGGG)4
Effect of γ-hydroxypropano deoxyguanosine, the major acrolein-derived adduct, on monomolecular quadruplex structure of telomeric repeat d(TTAGGG)4
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DOI：
10.1016/j.bmcl.2004.07.074
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发表时间：
2004-11-01
影响因子：
2.7
通讯作者：
Mayol, L
中科院分区：
文献类型：
--
作者：
D'Isa, G;Galeone, A;Mayol, L
合成了具有端粒重复序列d（TTAGGG）（4）并在不同位置掺入γ-羟基丙脱氧鸟苷的三种寡脱氧核糖核苷酸（ODNs）a-c。凝胶电泳和CD分析表明，ODNs假设在Na+和K+缓冲液中的单分子四链体结构。通过CD熔融实验获得的T-m值表明，丙烯醛-dG加合物进入ODN B中的存在降低了单分子四链体结构在Na+溶液中的热稳定性，而对于a和c，在相同的实验条件下没有检测到显著的影响。相反，所有ODN a-d在K+缓冲液中显示出相同的行为。这些调查结果进行了简要讨论。(C)2004 Elsevier Ltd.保留所有权利。
The three oligodeoxyribonucleotides (ODNs) a-c, having the telomeric repeat d(TTAGGG)(4) sequence and incorporating gamma-hydroxypropano deoxyguanosine at different positions, were synthesized. Gel electrophoresis and CD analyses indicated that the ODNs assume monomolecular quadruplex structures in Na+ and in K+ buffers. The T-m values, obtained by CD melting experiments, showed that the presence of the acrolein-dG adduct into the ODN b decreases the thermal stability of the monomolecular quadruplex structure in Na+ solution, whereas for a and c no significant effect could be detected in the same experimental conditions. On the contrary, all ODNs a-d show the same behaviour in K+ buffer. These findings are briefly discussed. (C) 2004 Elsevier Ltd. All rights reserved.