A role for DNA polymerase in gene conversion and crossing over during meiosis in Saccharomyces cerevisi"
A role for DNA polymerase in gene conversion and crossing over during meiosis in Saccharomyces cerevisi"
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DOI：
10.1534/genetics.104.026260
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发表时间：
2004-07-01
期刊：
影响因子：
3.3
通讯作者：
Roeder, GS
中科院分区：
文献类型：
--
作者：
Maloisel, L;Bhargava, J;Roeder, GS
对减数分裂基因转化缺陷的芽酵母突变株的筛选发现了POL3基因的一个新等位基因。POL3编码DNA聚合酶8的催化亚单位，DNA聚合酶8是参与基因组DNA复制的重要DNA聚合酶。新的等位基因pol3-ct指定了一种缺少最后四个氨基酸的蛋白质。Pol3-ct显示很少或没有DNA复制缺陷，但显示减数分裂基因转换区长度缩短和交叉减少。我们提出了一个模型，在这个模型中，DNA合成决定了链交换中间体的长度，并影响了它们对交换的分辨率。
A screen for mutants of budding yeast defective in meiotic gene conversion identified a novel allele of the POL3 gene. POL3 encodes the catalytic subunit of DNA polymerase 8, an essential DNA polymerase involved in genomic DNA replication. The new allele, pol3-ct, specifies a protein missing the last four amino acids. pol3-ct shows little or no defect in DNA replication, but displays a reduction in the length of meiotic gene conversion tracts and a decrease in crossing over. We propose a model in which DNA synthesis determines the length of strand exchange intermediates and influences their resolution toward crossing over.