ADAPTATION TO AND BIODEGRADATION OF XENOBIOTIC COMPOUNDS BY MICROBIAL COMMUNITIES FROM A PRISTINE AQUIFER
ADAPTATION TO AND BIODEGRADATION OF XENOBIOTIC COMPOUNDS BY MICROBIAL COMMUNITIES FROM A PRISTINE AQUIFER
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DOI：
10.1128/aem.53.9.2212-2217.1987
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发表时间：
1987-09-01
影响因子：
4.4
通讯作者：
PFAENDER, FK
中科院分区：
文献类型：
--
作者：
AELION, CM;SWINDOLL, CM;PFAENDER, FK
地下微生物群落的能力，以适应生物外源性化合物的生物降解进行了检查，在含水层固体样品从原始含水层。放射性标记底物的矿化率随着暴露而增加被用作适应的指标。对于一些化合物，如氯苯和1，2，4-三氯苯，轻微的矿化，但没有适应是明显的，在孵育超过8个月。其他化合物表现出三种反应模式。对于间甲酚，间氨基苯酚和苯胺的中间速率的生物降解和矿化百分比随时间的线性增加进行了观察。苯酚，对氯苯酚，和二溴乙烯迅速代谢最初，随着时间的推移，矿化的百分比非线性增加，表明该社区已经适应这些化合物的生物降解。只有对硝基苯酚表现出典型的适应反应。在同一含水层的不同土壤样品中，对对硝基苯酚的适应期从几天到长达6周不等。在大多数情况下，添加的外源性物质的浓度范围从每克几纳克到微克，对反应没有影响。最可能数计数表明，适应是伴随着特定的降解物数量的增加。这项研究表明，不同的模式的响应发生在地下微生物群落。
The ability of subsurface microbial communities to adapt to the biodegradation of xenobiotic compounds was examined in aquifer solids samples from a pristine aquifer. An increase in the rates of mineralization of radiolabeled substrates with exposure was used as an indication of adaptation. For some compounds, such as chlorobenzene and 1,2,4-trichlorobenzene, slight mineralization was observed but no adaptation was apparent during incubations for over 8 months. Other compounds demonstrated three patterns of response. For m-cresol, m-aminophenol, and aniline intermediate rates of biodegradation and a linear increase in the percent mineralized with time were observed. Phenol, p-chlorophenol, and ethylene dibromide were rapidly metabolized initially, with a nonlinear increase in the percent mineralized with time, indicating that the community was already adapted to the biodegradation of these compounds. Only p-nitrophenol demonstrated a typical adaptation response. In different samples of soil from the same layer in the aquifer, the adaptation period to p-nitrophenol varied from a few days to as long as 6 weeks. In most cases the concentration of xenobiotic added, over the range from a few nanograms to micrograms per gram, made no difference in the response. Most-probable-number counts demonstrated that adaptation is accompanied by an increase in specific degrader numbers. This study has shown that diverse patterns of response occur in the subsurface microbial community.