Regulation of herpesvirus macromolecular synthesis: transcription-initiation sites and domains of alpha genes.
Regulation of herpesvirus macromolecular synthesis: transcription-initiation sites and domains of alpha genes.
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疱疹病毒大分子合成的调节：转录起始位点和α基因的结构域。
DOI：
10.1073/pnas.77.12.7122
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发表时间：
1980
影响因子：
11.1
通讯作者：
Roizman,B
中科院分区：
文献类型：
--
作者：
Mackem,S;Roizman,B
单纯疱疹病毒1型（HSV-1）在感染细胞中指定一组多肽（α），其mrna是在缺乏蛋白质合成的情况下产生的。指定α多肽0、4、22和27的单个mrna通过杂交纯化到pBR322质粒中分离的HSV-1 DNA片段或总DNA片段，在体外翻译以验证其特异性，然后通过杂交定位到克隆DNA片段的分离链和酶切。为了绘制转录起始位点，用[α - 32p]GTP和牛痘病毒guananyltransferase重新盖住化学脱链的单个mrna，并将其与克隆DNA片段杂交。每个标记的5'末端杂交到一个特定的位点，两个在一条链上，两个在另一条链上。转录本的5'端不具有序列同源性，表明它们是从独立的启动子转录而来的。
Herpes simplex virus 1 (HSV-1) specifies in the infected cell a set of polypeptides (alpha) whose mRNAs are made in the absence of protein synthesis. The individual mRNAs specifying alpha polypeptides 0, 4, 22, and 27 were purified by hybridization to strand-separated or total HSV-1 DNA fragments cloned in pBR322 plasmids, translated in vitro to verify their specificity, then mapped by hybridization to separated strands and digests of cloned DNA fragments. To map the transcription initiation sites, chemically decapped individual mRNAs were recapped with [alpha-32P]GTP and vaccinia virus guanylyltransferase and hybridized to digests of the cloned DNA fragments. Each of the labeled 5' termini hybridized to a specific site, two on one strand, and two on the other. The 5' ends of the transcripts do not share sequence homology, suggesting that they are transcribed from independent promoters.