Shedding light on clock controlled cab gene transcription in higher plants.
Shedding light on clock controlled cab gene transcription in higher plants.
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DOI：
10.1006/scel.1993.1010
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发表时间：
1993-04
期刊：
影响因子：
--
通讯作者：
S. Kay
中科院分区：
文献类型：
--
作者：
S. Kay
几个实验室的研究表明，生物钟调节着许多高等植物基因的转录。这些基因中最具特征的是编码光系统II捕光复合体的叶绿素a/b结合蛋白(Cab)的基因。对黄化植物和绿色植物中CaB基因表达的分析表明，光敏色素(S)与生物钟之间存在复杂的相互作用，其中光敏色素似乎调节CaB转录水平，生物钟调节该阶段。一种基于萤火虫荧光素酶在转基因植物中表达的非侵入性CAB转录方法的建立，不仅使分析活植物中时钟调控的转录成为可能，而且也为筛选拟南芥中的时钟突变体提供了手段。
Research from several laboratories has revealed that the circadian clock regulates the transcription of many higher plant genes. Amongst the most well characterized of these genes are those that encode the chlorophyll a/b binding (Cab) proteins of the light harvesting complexes of photosystem II. Analysis of cab gene expression in both etiolated and green plants reveals a complex interaction between phytochrome(s) and the circadian clock, in which phytochrome appears to regulate the level of cab transcription and the circadian clock regulates the phase. The development of a non-invasive assay for cab transcription based on firefly luciferase expression in transgenic plants has not only made it possible to analyze clock regulated transcription in living plants, but also provides a means to screen for clock mutants in Arabidopsis.