A nonneural miRNA cluster mediates hearing via repression of two neural targets
A nonneural miRNA cluster mediates hearing via repression of two neural targets
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非神经 miRNA 簇通过抑制两个神经靶标介导听力
DOI：
10.1101/gad.351052.123
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发表时间：
2023
影响因子：
10.5
通讯作者：
Lai, Eric C.
中科院分区：
文献类型：
--
作者：
Zhang, Binglong;Duan, Hong;Kavaler, Joshua;Wei, Lu;Eberl, Daniel F.;Lai, Eric C.
我们发现mir-279/996对果蝇主要的本体感受和听觉器官约翰斯顿器官（JO）的发育和功能是绝对必要的。它们的缺失导致高度异常的细胞命运决定，包括髓样细胞和异位神经元的丢失，并且突变体是电生理学聋的。体内活性传感器和镶嵌分析表明，这些种子相关的miRNAs的功能自主抑制非神经元细胞的神经命运。最后，遗传相互作用确定了两个神经靶点（elav和insensitive），这两个靶点是miRNA突变型JO表型的基础。这项工作揭示了特定miRNA靶点的关键转录后调控如何控制听觉系统的细胞特异性和功能。
We show here that mir-279/996 are absolutely essential for development and function of Johnston's organ (JO), the primary proprioceptive and auditory organ in Drosophila. Their deletion results in highly aberrant cell fate determination, including loss of scolopale cells and ectopic neurons, and mutants are electrophysiologically deaf. In vivo activity sensors and mosaic analyses indicate that these seed-related miRNAs function autonomously to suppress neural fate in nonneuronal cells. Finally, genetic interactions pinpoint two neural targets (elav and insensible) that underlie miRNA mutant JO phenotypes. This work uncovers how critical post-transcriptional regulation of specific miRNA targets governs cell specification and function of the auditory system.