Luminous bacteria in the deep-sea waters near the ANTARES underwater neutrino telescope (Mediterranean Sea)
Luminous bacteria in the deep-sea waters near the ANTARES underwater neutrino telescope (Mediterranean Sea)
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DOI：
10.1080/02757540903513766
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发表时间：
2010-01-01
影响因子：
2.5
通讯作者：
Tamburini, Christian
中科院分区：
文献类型：
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作者：
Al Ali, Badr;Garel, Marc;Tamburini, Christian
海洋发光深海细菌可能是安装在地中海深度为2000至2475米的Antares中微子望远镜的一个潜在信号微扰源。利用卡片-FISH方法，我们通过Antares站点附近的水柱估计了细菌、变形杆菌和弧菌科(海洋发光细菌的属、纲和科)的总原核生物的相对丰度。在2200米深处，弧菌科似乎远远不可忽视，占变形杆菌的40%，细菌的25%，DAPI染色细胞总数的9%，而细菌和古生菌各占35%。此外，在中微子望远镜探测到的高发光本底期间，我们从2200米深的样品中分离到一株嗜压发光细菌，经系统发育鉴定为发光发光菌ANT-2200。我们已经利用该菌株通过开发一种新的高压装置来研究静水压力对生物发光的影响。初步测定表明，发光菌菌株ANT-2200在22 Mpa时的发光强度是0.1 Mpa(大气压)时的5倍。
Marine luminous deep-sea bacteria may represent a potential source of signal perturbation for the ANTARES neutrino telescope installed between 2000 and 2475 m depth in the Mediterranean Sea. Using the CARD-FISH method, we have estimated the relative abundances of total prokaryotes of Bacteria, -proteobacteria and Vibrinoceae (domain, class and family affiliation of marine luminous bacteria, respectively) through the water column close to the ANTARES site. At 2200 m depth, Vibrionaceae appeared to be far from negligible, representing 40% of -proteobacteria, 25% of Bacteria and 9% of the total DAPI-stained cells, while Bacteria and Archaea represented 35% each. Furthermore, during a high luminous background period detected by the neutrino telescope, we isolated, from a 2200 m depth sample, a piezophilic luminous bacterium, phylogenetically determined as Photobacterium phosphoreum strain ANT-2200. We have used this strain to investigate the effect of hydrostatic pressure on bioluminescence by developing a new high-pressure apparatus. First assays showed that the bioluminescence intensity of Photobacterium phosphoreum strain ANT-2200 was 5 times higher at 22MPa than at 0.1MPa (atmospheric pressure).