Expression and hypoxic regulation of hif1alpha and hif2alpha during early blood and endothelial cell differentiation in chick.
Expression and hypoxic regulation of hif1alpha and hif2alpha during early blood and endothelial cell differentiation in chick.
复制标题
鸡早期血液和内皮细胞分化过程中 hif1α 和 hif2α 的表达和缺氧调节。
DOI：
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发表时间：
2007
影响因子：
1.2
通讯作者：
Guojun Sheng
中科院分区：
文献类型：
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作者：
K. Ota;Hiroki Nagai;Guojun Sheng
HIF 1和HIF 2是缺氧感知和反应的主要介质。它们在红细胞中参与氧气供应的两种关键细胞类型（原始血细胞和内皮细胞）的早期分化中的作用尚不清楚。我们发现，在循环前的禽类胚胎中，hif1 α和hif2 α分别在胚胎和胚外组织中表达。HIF 2 α首先被鉴定为epas1，在常氧或缺氧条件下，在任何循环前阶段都不存在于内皮细胞中。分化中的血细胞在常氧下表达低水平的hif2 α，但在缺氧下表现出强烈和快速的上调。然而，血细胞分化在缺氧或高氧条件下均不受影响。
HIF1 and HIF2 are major mediators for hypoxia sensing and response. Their roles in early differentiation of two key cell types involved in oxygen supply in amniotes, the primitive blood cells and endothelial cells, are unclear. We show that, in pre-circulation avian embryos, hif1alpha and hif2alpha are expressed in embryonic and extraembryonic tissues, respectively. hif2alpha, first identified as epas1, is not present in endothelial cells at any pre-circulation stage under either normoxia or hypoxia conditions. Differentiating blood cells express low levels of hif2alpha under normoxia, but show a strong and rapid upregulation under hypoxia. Blood cell differentiation, however, is not affected under either hypoxia or hyperoxia conditions.