Gamma-protocadherin homophilic interaction and intracellular trafficking is controlled by the cytoplasmic domain in neurons.
Gamma-protocadherin homophilic interaction and intracellular trafficking is controlled by the cytoplasmic domain in neurons.
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DOI：
10.1016/j.mcn.2008.12.002
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发表时间：
2009-03
期刊：
影响因子：
--
通讯作者：
Phillips GR
中科院分区：
文献类型：
--
作者：
Fernández-Monreal M;Kang S;Phillips GR
γ -原钙粘蛋白（Pcdh-γ - s）是介导突触发生特异性的良好候选物质，但其在细胞-细胞相互作用中的作用仍存在争议。我们提出Pcdh-γ - s通过运输含有Pcdh-γ - s的细胞器，插入到突触膜和亲同性的跨细胞相互作用来修饰预形成的突触。在这里，我们提供证据来支持这个模型。我们首次发现pdhh -γ - s具有亲同性，并且在神经元发育过程中在树突和轴-树突界面积累。Pcdh-γ - s维持在树突内的大量移动胞内池中，细胞质缺失将分子转移到表面，减少了移动包的数量和速度。我们监测了Pcdh-γ在运输细胞器中的时空动态。pdhh -γ细胞器与突触附近的固定簇萌发和融合。这些结果表明，pdhh -γ介导的突触发育或维持中的细胞间相互作用受到胞质域胞内运输控制的严格调节。
Gamma-protocadherins (Pcdh-γs) are good candidates to mediate specificity in synaptogenesis but their role in cell-cell interactions is a matter of debate. We proposed that Pcdh-γs modify preformed synapses via trafficking of Pcdh-γs-containing organelles, insertion into synaptic membranes and homophilic transcellular interaction. Here we provide evidence in support of this model. We show for the first time that Pcdh-γs have homophilic properties and that they accumulate at dendrodendritic and axo-dendritic interfaces during neuronal development. Pcdh-γs are maintained in a substantial mobile intracellular pool in dendrites and cytoplasmic deletion shifts the molecule to the surface and reduces the number and velocity of the mobile packets. We monitored Pcdh-γ temporal and spatial dynamics in transport organelles. Pcdh-γ organelles bud and fuse with stationary clusters near synapses. These results suggest that Pcdh-γ-mediated cell-cell interactions in synapse development or maintenance are tightly regulated by control of intracellular trafficking via the cytoplasmic domain.
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