Molecular structure of fd (f1, M13) filamentous bacteriophage refined with respect to X-ray fibre diffraction and solid-state NMR data supports specific models of phage assembly at the bacterial membrane
Molecular structure of fd (f1, M13) filamentous bacteriophage refined with respect to X-ray fibre diffraction and solid-state NMR data supports specific models of phage assembly at the bacterial membrane
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DOI：
10.1016/j.jmb.2005.10.048
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发表时间：
2006-01-13
影响因子：
5.6
通讯作者：
Straus, SK
中科院分区：
文献类型：
--
作者：
Marvin, DA;Welsh, LC;Straus, SK
丝状噬菌体（Inovirus）是一种简单且特性良好的模型系统。噬菌体颗粒或病毒粒子直径约为60a，长数千埃。当病毒粒子挤出宿主而不杀死宿主时，它们在细菌膜上组装，这是核蛋白组装跨膜特定运输的一个例子。Ff基团（fd, f1）尤其是M13)被广泛研究。基于x射线纤维衍射得到的fd病毒粒子的分子模型，提出了病毒粒子组装的模型。利用固态核磁共振数据提出了一种稍微不同的fd病毒粒子模型，与这些组装模型和x射线衍射数据不一致。在这里，我们表明重新解释的核磁共振数据也与x射线纤维衍射研究得出的模型一致，并讨论了病毒粒子组装的模型。(c) 2005 Elsevier Ltd版权所有。
Filamentous bacteriophage (Inovirus) is a simple and well-characterized model system. The phage particle, or virion, is about 60 A in diameter and several thousand angstrom units long. The virions are assembled at the bacterial membrane as they extrude out of the host without killing it, an example of specific transport of nucleoprotein assemblages across membranes. The Ff group (fd, f1. and M13) has been especially widely studied. Models of virion assembly have been proposed based on a molecular model of the fd virion derived by X-ray fibre diffraction. A somewhat different model of the fd virion using solid-state NMR data has been proposed, not consistent with these models of assembly nor with the X-ray diffraction data. Here we show that reinterpreted NMR data are also consistent with the model derived from X-ray fibre diffraction studies, and discuss models of virion assembly. (c) 2005 Elsevier Ltd. All rights reserved.